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| Research article summary (published 30 Jul 2009): |
Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC.
Full Abstract
RP HPLC-UV-MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 microg/mL for myricetin and quercetin and 0.5 microg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5-3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1-20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process.
Author information
Author/s: Biesaga, Magdalena (M); Ochnik, Urszula (U); Pyrzynska, Krystyna (K);
Affiliation: Department of Chemistry, University of Warsaw, Warsaw, Poland.
Journal and publication information
Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
Journal: Journal of separation science (J Sep Sci), published in Germany. (Language: eng)
Reference: 2009-Aug; vol 32 (issue 15-16) : pp 2835-40
Dates: Created 2009/08/17; Completed 2009/10/22;
PMID: 19575378, status: MEDLINE (last retrieved date: 10/22/2009)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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MeSH headings (categories)
This article was linked to the MeSH Headings (categories) shown below.
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Associated Chemicals: Flavanones (0) ; Flavonoids (0) ; Kaempferols (0) ; Plant Extracts (0) ; naringin (10236-47-2) ; Quercetin (117-39-5) ; Rutin (153-18-4) ; naringenin (480-41-1) ; kaempferol (520-18-3) ; myricetin (529-44-2) ; Methanol (67-56-1)Related articles
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