Research article summary (published 13 Oct 2009):

Reduced expression and homozygous deletion of annexin A10 in gastric carcinoma.

Full Abstract

Annexins (ANXs) are a family of calcium and phospholipid binding proteins that have been implicated in diverse important biological and physiological process. ANX A10 (ANXA10) is a member of this family, though little is known about its functions. In the present study, array based comparative genomic hybridization (CGH) was used to screen DNA copy number change in gastric cancer cell lines and the results obtained were compared with oligonucleotide microarray data. DNA loss of the ANXA10 locus in chromosome 4q33 was found in several gastric cancer cell lines by array based CGH and these cell lines showed decreased ANXA10 expression by oligonucleotide microarray analysis. Functional analysis using siRNA and full-length cDNA transfection in gastric cancer cell lines demonstrated that ANXA10 regulates gastric cancer cell proliferation. Of the 585 primary gastric carcinoma tissues examined, ANXA10 expression at the protein level was found to be reduced in 289 (49.4%) cases. Quantitative real-time PCR analysis validated loss of DNA at the ANXA10 locus in gastric carcinomas with reduced ANXA10 expression. By univariate survival analysis, lack of ANXA10 expression was associated with poor survival (p = 0.016). These results suggest that ANXA10 inactivation by chromosomal deletion may play a role during gastric cancer progression.

Author information

Author/s: Kim, Jin (J); Kim, Min A (MA); Jee, Chang Do (CD); Jung, Eun Ji (EJ); Kim, Woo Ho (WH);

Affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul 110-799, Korea.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Oct; vol 125 (issue 8) : pp 1842-50

Dates: Created 2009/08/26; Completed 2009/09/21;

PMID: 19582876, status: MEDLINE (last retrieval date: 9/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenocarcinoma - genetics; metabolism; secondary Annexins - antagonists & inhibitors; genetics; metabolism Apoptosis Blotting, Western Case-Control Studies Cell Movement Cell Proliferation Cells, Cultured Chromosome Mapping Chromosomes, Human, Pair 4 - genetics Colony-Forming Units Assay Comparative Genomic Hybridization Female Gene Deletion Gene Expression Profiling Homozygote Humans Immunoblotting

Immunoblotting Immunoenzyme Techniques In Situ Hybridization, Fluorescence Kidney - embryology; metabolism; pathology Lymphatic Metastasis Male Mutation - genetics Neoplasm Staging Oligonucleotide Array Sequence Analysis Prognosis RNA, Messenger - genetics; metabolism RNA, Small Interfering - pharmacology Reverse Transcriptase Polymerase Chain Reaction Stomach - metabolism; pathology Stomach Neoplasms - genetics; metabolism; pathology Tissue Array Analysis Tumor Cells, Cultured Wound Healing

Associated Chemicals: ANXA10 protein, human (0) ; Annexins (0) ; RNA, Messenger (0) ; RNA, Small Interfering (0)

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