Research article summary (published 30 Oct 2009):

Functional role of aquaglyceroporin 7 expression in the pancreatic beta-cell line BRIN-BD11.

Full Abstract

Both mouse and rat pancreatic islet beta-cells were recently found to express aquaglyceroporin 7 (AQP7). In the present study, the expression and role of AQP7 in the function of BRIN-BD11 cells were investigated. AQP7 mRNA and protein were detected by RT-PCR and Western blot analysis, respectively. In an isoosmolar medium, the net uptake of [2-(3)H]glycerol displayed an exponential time course reaching an equilibrium plateau value close to its extracellular concentration. Within 2 min of incubation in a hypotonic medium (caused by a 50 mM decrease in NaCl concentration), the [2-(3)H]glycerol uptake averaged 143.2 +/- 3.8% (n = 24; P < 0.001) of its control value in isotonic medium, declining thereafter consistently with previously demonstrated volume regulatory decrease. When isoosmolarity was restored by the addition of 100 mM urea to the hypotonic medium, [2-(3)H]glycerol uptake remained higher (112.1 +/- 2.8%, n = 24; P < 0.001) than its matched control under isotonic conditions, indicating rapid entry of urea and water. Insulin release by BRIN-BD11 cells was 3 times higher in hypotonic than in isotonic medium. When glycerol (100 mM) or urea (100 mM) were incorporated in the hypotonic medium, the insulin release remained significantly higher than that found in the control isotonic medium, averaging respectively 120.2 +/- 4.2 and 107.0 +/- 3.8% of the paired value recorded in the hypotonic medium. These findings document the rapid entry of glycerol and urea in BRIN-BD11 cells, likely mediated by AQP7.

Author information

Author/s: Delporte, C (C); Virreira, M (M); Crutzen, R (R); Louchami, K (K); Sener, A (A); Malaisse, W J (WJ); Beauwens, R (R);

Affiliation: Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of cellular physiology (J Cell Physiol), published in United States. (Language: eng)

Reference: 2009-Nov; vol 221 (issue 2) : pp 424-9

Dates: Created 2009/08/27; Completed 2009/09/09;

PMID: 19585522, status: MEDLINE (last retrieval date: 9/9/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Aquaporins - genetics; metabolism Cell Line Gene Expression Regulation - drug effects Glycerol - metabolism Insulin - secretion Insulin-Secreting Cells - drug effects; metabolism; secretion

Mercuric Chloride - pharmacology RNA, Messenger - genetics; metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Sulfadiazine - pharmacology Time Factors Urea - pharmacology

Associated Chemicals: Aqp7 protein, rat (0) ; Aquaporins (0) ; RNA, Messenger (0) ; Insulin (11061-68-0) ; Glycerol (56-81-5) ; Urea (57-13-6) ; Sulfadiazine (68-35-9) ; Mercuric Chloride (7487-94-7)

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