Skin and peripheral lymph node invariant NKT cells are mainly retinoic acid receptor-related orphan receptor (gamma)t+ and respond preferentially under inflammatory conditions.

Full Abstract

Lymph nodes (LNs) have been long considered as comprising few invariant NKT (iNKT) cells, and these cells have not been studied extensively. In this study, we unravel the existence of stable rather than transitional LN-resident NK1.1(-) iNKT cell populations. We found the one resident in peripheral LNs (PLNs) to comprise a major IL-17-producing population and to express the retinoic acid receptor-related orphan receptor (gamma)t (ROR(gamma)t). These cells respond to their ligand alpha-galactosylceramide (alpha-GalCer) in vivo by expanding dramatically in the presence of LPS, providing insight into how this rare population could have an impact in immune responses to infection. PLN-resident ROR(gamma)t(+) NK1.1(-) iNKT cells express concomitantly CCR6, the integrin alpha-chain alpha(E) (CD103), and IL-1R type I (CD121a), indicating that they might play a role in inflamed epithelia. Accordingly, skin epithelia comprise a major ROR(gamma)t(+) CCR6(+)CD103(+)CD121a(+) NK1.1(-) cell population, reflecting iNKT cell composition in PLNs. Importantly, both skin and draining PLN ROR(gamma)t(+) iNKT cells respond preferentially to inflammatory signals and independently of IL-6, indicating that they could play a nonredundant role during inflammation. Overall, our study indicates that ROR(gamma)t(+) iNKT cells could play a major role in the skin during immune responses to infection and autoimmunity.

Author information

Author/s: Doisne, Jean-Marc (JM); Becourt, Chantal (C); Amniai, Latiffa (L); Duarte, Nadia (N); Le Luduec, Jean-Benoît (JB); Eberl, Gérard (G); Benlagha, Kamel (K);

Affiliation: INSERM Unité 561/Groupe AVENIR, Hôpital Cochin St Vincent de Paul, Université Descartes, Paris, France.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol), published in United States. (Language: eng)

Reference: 2009-Aug; vol 183 (issue 3) : pp 2142-9

Dates: Created 2009/07/21; Completed 2009/08/06;

PMID: 19587013, status: MEDLINE (last retrieved date: 8/21/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals
Cell Proliferation - drug effects
Epithelial Cells - pathology
Galactosylceramides - pharmacology
Inflammation - immunology; pathology
Interleukin-17
Interleukin-6 - deficiency

Lymph Nodes - cytology; pathology
Mice
Mice, Knockout
Natural Killer T-Cells - cytology; immunology
Receptors, Retinoic Acid
Receptors, Thyroid Hormone
Skin - cytology; pathology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Galactosylceramides (0) ; Interleukin-17 (0) ; Interleukin-6 (0) ; Receptors, Retinoic Acid (0) ; Receptors, Thyroid Hormone (0) ; alpha-galactosylceramide (0) ; orphan nuclear receptor ROR-gamma (0)

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