Research article summary (published 25 Jun 2009):

Inhibitory effects of flavonoids isolated from Fragaria ananassa Duch on IgE-mediated degranulation in rat basophilic leukemia RBL-2H3.

Full Abstract

We isolated the 4 kinds of flavonoids from strawberry 'Nohime' and examined the effect of these flavonoids on the degranulation in RBL-2H3 cells. The flavonoids were found to suppress the degranulation from Ag-stimulated RBL-2H3 cells to different extents. To disclose the inhibitory mechanism of degranulation by flavonoids, we examined their effects on the intracellular free Ca(2+) concentration ([Ca(2+)]i) and the intracellular signaling pathway such as Lyn, Syk, and PLCgammas. The intracellular free Ca(2+) concentration ([Ca(2+)]i) was elevated by Fc epsilonRI activation, but these flavonoid treatments reduced the elevation of [Ca(2+)]i by suppressing Ca(2+) influx. Kaempferol strongly suppressed the activation of Syk and PLCgammas. It was thus suggested that suppression of Ag-stimulated degranulation by the flavonoids is mainly due to suppression of [Ca(2+)]i elevation and Syk activation. These results suggested that strawberry would be of some ameliorative benefit for the allergic symptoms.

Author information

Author/s: Itoh, Tomohiro (T); Ninomiya, Masayuki (M); Yasuda, Masaharu (M); Koshikawa, Kaneyuki (K); Deyashiki, Yoshihiro (Y); Nozawa, Yoshinori (Y); Akao, Yukihiro (Y); Koketsu, Mamoru (M);

Affiliation: Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. titoh(-atsign-)giib.or.jp

Journal and publication information

Publication Type: Journal Article

Journal: Bioorganic & medicinal chemistry (Bioorg Med Chem), published in England. (Language: eng)

Reference: 2009-Aug; vol 17 (issue 15) : pp 5374-9

Dates: Created 2009/07/21; Completed 2009/10/13;

PMID: 19596200, status: MEDLINE (last retrieval date: 10/13/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Anti-Allergic Agents - analysis; isolation & purification; pharmacology Basophil Degranulation Test Basophils - cytology; drug effects; enzymology; immunology Calcium - metabolism Cell Degranulation - drug effects Cell Line, Tumor Flavonoids - analysis; isolation & purification; pharmacology Fragaria - chemistry Fruit - chemistry

Immunoglobulin E - immunology Intracellular Signaling Peptides and Proteins - metabolism Leukemia - enzymology; immunology Molecular Structure Protein-Tyrosine Kinases - metabolism Rats Reactive Oxygen Species - metabolism Receptors, IgE - immunology Signal Transduction - drug effects beta-N-Acetylhexosaminidases - antagonists & inhibitors; metabolism

Associated Chemicals: Anti-Allergic Agents (0) ; Flavonoids (0) ; Intracellular Signaling Peptides and Proteins (0) ; Reactive Oxygen Species (0) ; Receptors, IgE (0) ; Immunoglobulin E (37341-29-0) ; Calcium (7440-70-2) ; Protein-Tyrosine Kinases (EC 2.7.1.112) ; Syk kinase (EC 2.7.1.112) ; beta-N-Acetylhexosaminidases (EC 3.2.1.52)

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