Heat-shock pretreatment inhibits sorbitol-induced apoptosis in K562, U937 and HeLa cells.

Full Abstract

The aim of this study was to determine whether heat-shock pretreatment exerted a protective effect against sorbitol-induced apoptotic cell death in K562, U937 and HeLa cell lines and whether such protection was associated with a decreased cytochrome c release from mithocondria and a decreased activation of caspase-9 and -3. Following heat-shock pretreatment (42 +/- 0.3 degrees C for 1 hr), these cell lines were exposed to sorbitol for 1 hr. Apoptosis was evaluated by DNA fragmentation, whereas caspase-9,-3 activation, cytochrome c release and heat-shock protein70 (HSP70) were assayed by Western Blot. Sorbitol exposure-induced apoptosis in these different cell lines with a marked activation of caspase-9 and caspase-3, whereas heat-shock pretreatment before sorbitol exposure, induced expression of HSP70 and inhibited sorbitol-mediated cytochrome c release and subsequent activation of caspase-9 and caspase-3. Similarly, overexpression of HSP70 in the three cell lines studied prevented caspase-9 cleavage and activation as well as cell death. Furthermore, we showed that the mRNA expression of iNOS decreased during both the heat-shock treatment and heat-shock pretreatment before sorbitol exposure. By contrast, the expression of Cu-Zn superoxide dismutase (SOD) and Mn-SOD proteins increased during heat-shock pretreatment before sorbitol exposure. We conclude that, heat-shock pretreatment protects different cell lines against sorbitol-induced apoptosis through a mechanism that is likely to involve SOD family members. (c) 2009 UICC.

Author information

Author/s: Marfe, Gabriella (G); Pucci, Bruna (B); De Martino, Luisa (L); Fiorito, Filomena (F); Di Stefano, Carla (C); Indelicato, Manuela (M); Aventaggiato, Michele (M); Russo, Matteo A (MA); Tafani, Marco (M);

Affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome "Tor Vergata", Via Montpellier 1, Rome, Italy. gabriellamarfe(-atsign-)libero.it

Journal and publication information

Publication Type: Journal Article

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Nov; vol 125 (issue 9) : pp 2077-85

Dates: Created 2009/09/03; Completed 2009/09/15;

PMID: 19598258, status: MEDLINE (last retrieval date: 9/15/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Apoptosis - drug effects
Caspase 3 - metabolism
Caspase 9 - metabolism
Cytochromes c - secretion
DNA Fragmentation
HSP70 Heat-Shock Proteins - biosynthesis
Hela Cells
Hot Temperature
Humans

Associated Chemicals: BAX protein, human (0) ; HSP70 Heat-Shock Proteins (0) ; Proto-Oncogene Proteins c-bcl-2 (0) ; RNA, Messenger (0) ; Reactive Oxygen Species (0) ; bcl-2-Associated X Protein (0) ; Sorbitol (50-70-4) ; Cytochromes c (9007-43-6) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Superoxide Dismutase (EC 1.15.1.1) ; Caspase 3 (EC 3.4.22.-) ; Caspase 9 (EC 3.4.22.-)

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