Research article summary (published 30 Jul 2009):

Immunostimulatory properties of coffee mannans.

Full Abstract

Coffee infusion mannans are acetylated polysaccharides containing single Galp and Araf residues as side chains of a beta-(1 --> 4)-Manp backbone. These mannans are structurally similar to the bioactive acetylated mannans from Aloe vera (AV). In this study, acetylated mannans were obtained from two coffee infusions prepared from light and dark roasted beans. These samples were tested for their immunostimulatory activity and compared with an extract of AV mannan and with locust bean gum (LBG) galactomannans. The coffee samples, as well as the AV extract, stimulated murine B- and T-lymphocytes, as evaluated by the in vitro expression of the surface lymphocyte activation marker CD69, more marked on B- than on T-lymphocytes. In coffee samples, contrarily to the AV, no proliferative effect was noticed. LBG sample did not show any immunostimulatory activity. Because the material that remains in the residue of the hot water extraction was still very rich in mannans, a sequential extraction was performed and a main fraction was recovered with a 4 M NaOH solution. Because this material was insoluble in water, a partial acetylation was performed. These polysaccharides also showed immunostimulatory activity, opening the possibility of exploitation of coffee infusion and coffee residue as sources of bioactive polysaccharides.

Author information

Author/s: Simões, Joana (J); Madureira, Pedro (P); Nunes, Fernando M (FM); Domingues, Maria do Rosário (Mdo R); Vilanova, Manuel (M); Coimbra, Manuel A (MA);

Affiliation: Departamento de Química, Universidade de Aveiro, 3810- 193 Aveiro, Portugal.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Molecular nutrition & food research (Mol Nutr Food Res), published in Germany. (Language: eng)

Reference: 2009-Aug; vol 53 (issue 8) : pp 1036-43

Dates: Created 2009/08/03; Completed 2009/10/14;

PMID: 19603397, status: MEDLINE (last retrieval date: 10/14/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetylation Adjuvants, Immunologic - pharmacology Aloe - chemistry Animals Coffee - chemistry

Lymphocyte Activation - drug effects Male Mannans - pharmacology Mice Mice, Inbred C57BL

Associated Chemicals: Adjuvants, Immunologic (0) ; Coffee (0) ; Mannans (0)

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