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| Research article summary (published 29 Sep 2009): |
Objective color scale for the SWYPE surface sampling technique using computerized image analysis tools.
Full Abstract
Colorimetric SWYPE pads are useful tools for identifying unpolymerized aliphatic isocyanates on a variety of surfaces. This technique has been used in autobody shops to determine the presence of hexamethylene diisocyanate and other aliphatic isocyanates that are important constituents of many automotive coatings. SWYPEs have the advantage of being relatively inexpensive, rapid, and portable. The color change elicited by aliphatic isocyanates (from yellow to red) provides a visual indication of the extent of surface contamination. To quantify isocyanate contamination based on the colorimetric response, an objective color scale was developed for isocyanate loading. Sampled pads were digitized on a calibrated, portable flatbed scanner, and red-green-blue (RGB) histograms of SWYPE images were created. A calibration curve was created from a series of reference images derived from SWYPEs loaded with an isocyanate-containing product. The SWYPE RGB analysis allowed for quantification over a range of isocyanate loadings: from approximately 0.01 to 24.0 microg/cm(2), with reproducibility of >90%, accuracy >90%, and a surface extraction efficiency of >90%. RGB analysis exhibited a lower detection limit than visual scoring (approximately 3 microg versus approximately 10 microg). The colorimetric response was cross validated with a high-performance liquid chromatography quantitative assay. When combined with RGB analysis, SWYPE colorimetric wipes represent a rapid and inexpensive method to assess objectively surface contamination with aliphatic isocyanates.
Author information
Author/s: Ceballos, Diana M (DM); Yost, Michael G (MG); Whittaker, Stephen G (SG); Camp, Janice (J); Dills, Russell (R);
Affiliation: Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195-7234, USA.
Journal and publication information
Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.; Validation Studies
Journal: Journal of occupational and environmental hygiene (J Occup Environ Hyg), published in United States. (Language: eng)
Reference: 2009-Oct; vol 6 (issue 10) : pp 604-11
Dates: Created 2009/07/16; Completed 2009/09/25;
PMID: 19606376, status: MEDLINE (last retrieval date: 9/25/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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