Research article summary (published 30 Jul 2009):

Preparation of a poly(N-isopropylacrylamide-co-ethylene dimethacrylate) monolithic capillary and its application for in-tube solid-phase microextraction coupled to high-performance liquid chromatography.

Full Abstract

A poly(N-isopropylacrylamide-co-ethylene dimethacrylate) (poly(NIPAAm-co-EDMA)) monolith was in situ prepared in the capillary and was investigated for in-tube solid-phase microextraction (SPME). NIPAAm, an acrylamide monomer with isopropyl group, was crosslinked with EDMA. PEG of 400-20,000 Da molecular weight and methanol were selected as the binary porogens. The porous structures of the resulting monoliths have been assessed by SEM, nitrogen adsorption-desorption, and pressure drop measurements. To investigate the extraction mechanism, several groups of model analytes (including neutral, acidic, and basic) were examined. The result showed that this monolithic material exhibited high extraction efficiencies for compounds under highly acidic and basic conditions, which was due to the hydrophobic interactions and excellent pH stability of the monolith. The equilibrium extraction time profiles were also monitored for model compounds to evaluate the extraction capacity of monolithic capillary. Finally, the developed monolith in-tube SPME-HPLC method was applied to the determination of three tricyclic antidepressants from urine samples.

Author information

Author/s: Ma, Qiao (Q); Chen, Ming (M); Shi, Zhi-Guo (ZG); Feng, Yu-Qi (YQ);

Affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, PR China.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of separation science (J Sep Sci), published in Germany. (Language: eng)

Reference: 2009-Aug; vol 32 (issue 15-16) : pp 2592-600

Dates: Created 2009/08/17; Completed 2009/10/22;

PMID: 19606442, status: MEDLINE (last retrieved date: 10/22/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Acrylamides - chemistry Antidepressive Agents, Tricyclic - isolation & purification; urine Chromatography, High Pressure Liquid - instrumentation; methods Cross-Linking Reagents - chemistry Humans Hydrogen-Ion Concentration

Methacrylates - chemistry Molecular Weight Polyethylene Glycols - chemistry Porosity Reproducibility of Results Solid Phase Microextraction - instrumentation; methods

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Acrylamides (0) ; Antidepressive Agents, Tricyclic (0) ; Cross-Linking Reagents (0) ; Methacrylates (0) ; Polyethylene Glycols (0) ; N-isopropylacrylamide (2210-25-5) ; ethylene dimethacrylate (97-90-5)

Related articles

These are the most related articles currently in our database:

• Electrophoresis gel media: the state of the art. 8 Oct 1997
• Uniformly sized molecularly imprinted polymer for atropine and its application to the determination of atropine and scopolamine in pharmaceutical preparations containing Scopolia extract. 21 Feb 2005
• Solid-phase microextraction-liquid chromatography (SPME-LC) determination of fluoxetine and norfluoxetine in plasma using a heated liquid flow through interface. 30 Oct 2006
• Thermosensitivity of N-isopropylacrylamide hydrogels cross-linked with degradable cross-linker. 30 Dec 2007
• Solid phase microextraction of macrolide, trimethoprim, and sulfonamide antibiotics in wastewaters. 30 Aug 2007
• Determination of fluoroquinolones in eggs using in-tube solid-phase microextraction coupled to high-performance liquid chromatography. 31 Jan 2006
• Poly(ethylene glycol)methacrylate-acrylamide copolymer media for hydrophobic protein and low temperature electrophoresis. 30 Jan 1994
• Plum-pudding gels as a platform for drug delivery: understanding the effects of the different components on the diffusion behavior of solutes. 3 Jun 2007
• Preparation and characterization of methacrylate-based semi-micro monoliths for high-throughput bioanalysis. 8 May 2006
• Preparation and evaluation of hydroxylated poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary for in-tube solid-phase microextraction coupled to high-performance liquid chromatography. 29 May 2007

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a larger map of 100+ related articles.