Research article summary (published 29 Nov 2009):

Downregulation of p21-activated kinase-1 inhibits the growth of gastric cancer cells involving cyclin B1.

Full Abstract

p21-Activated kinase 1 (Pak1), a serine/threonine kinase, has been implicated in cytoskeletal remodelling, cell motility, apoptosis and transformation. However, the role of Pak1 in gastric cancer remains unclear. In this study, we detected Pak1 expression in gastric cancer tissues from 40 patients by western blot. Overexpression of Pak1 was associated with progression, metastasis and prognosis of gastric cancer. In addition, we found that knockdown of Pak1 expression significantly inhibited anchorage-dependent and anchorage-independent growth in gastric cancer cells, and markedly inhibited gastric cancer cell xenograft tumor growth. In further study, data showed that Pak1 could regulate the expression of cyclin B1 at the mRNA and protein levels, and impact the subcellular distribution and the promoter activity of cyclin B1. Results from deletion and mutant analysis supplied a new NF-kappaB binding sites at position -321 of cyclin B1 promoter, and indicated that Pak1 regulated the transcription of cyclin B1 in gastric cancer through NF-kappaB. In conclusion, Pak1 may be a potential prognostic marker and therapeutic target in gastric cancer.

Author information

Author/s: Liu, Funan (F); Li, Xiaodong (X); Wang, Chunyu (C); Cai, Xinze (X); Du, Zhongmin (Z); Xu, Huimian (H); Li, Feng (F);

Affiliation: Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning, People's Republic of China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Dec; vol 125 (issue 11) : pp 2511-9

Dates: Created 2009/10/05; Completed 2009/11/06;

PMID: 19610058, status: MEDLINE (last retrieval date: 11/6/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Blotting, Northern Blotting, Western Cell Cycle Cell Proliferation Cyclin B - genetics; metabolism Down-Regulation Female Fluorescent Antibody Technique Humans Immunoenzyme Techniques

Lymphatic Metastasis Mice Mice, Nude NF-kappa B - genetics; metabolism Promoter Regions, Genetic - genetics RNA, Messenger - genetics; metabolism RNA, Small Interfering - pharmacology Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Stomach Neoplasms - metabolism; pathology p21-Activated Kinases - antagonists & inhibitors; metabolism

Associated Chemicals: Cyclin B (0) ; NF-kappa B (0) ; Pak1 protein, mouse (0) ; RNA, Messenger (0) ; RNA, Small Interfering (0) ; cyclin B1 (0) ; PAK1 protein, human (EC 2.7.11.1) ; p21-Activated Kinases (EC 2.7.11.1)

Related articles

These are the highest related articles currently in the database:

• KCNE2, a down-regulated gene identified by in silico analysis, suppressed proliferation of gastric cancer cells. 2 May 2006
• Identification and characterization of a novel p42.3 gene as tumor-specific and mitosis phase-dependent expression in gastric cancer. 19 May 2007
• Helicobacter pylori inhibits activity of cdc2 kinase and delays G2/M to G1 progression in gastric adenocarcinoma cell line. 30 Jan 2003
• Induction of G2/M arrest and apoptosis by water extract of Strychni Semen in human gastric carcinoma AGS cells. 30 May 2008
• Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer. 13 Nov 2004
• [Analysis of gene expression profiles in gastric cancer cell cycle] 30 Jul 2006
• Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer. 9 Apr 2006
• Overexpression of cyclin B1 in gastric cancer and its clinicopathological significance: an immunohistological study. 24 Jul 2002
• Methylation of the calcium channel-related gene, CACNA2D3, is frequent and a poor prognostic factor in gastric cancer. 14 May 2008
• Inhibitory effect of Polo-like kinase 1 depletion on mitosis and apoptosis of gastric cancer cells. 5 Jan 2006

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.