Research article summary (published 12 Sep 2009):

Development of Dictyostelium discoideum is associated with alteration of fucosylated N-glycan structures.

Full Abstract

The social amoeba Dictyostelium discoideum has become established as a simple model for the examination of cell-cell interactions, and early studies suggested that shifts in glycosylation profiles take place during its life cycle. In the present study, we have applied HPLC and mass spectrometric methods to show that the major N-glycans in axenic cultures of the AX3 strain are oligomannosidic forms, most of which carry core fucose and/or intersecting and bisecting N-acetylglucosamine residues, including the major structure with the composition Man8GlcNAc4Fuc1. The postulated alpha1,3-linkage of the core fucose correlates with the cross-reactivity of Dictyostelium glycoproteins with a horseradish peroxidase antiserum; a corresponding core alpha1,3-fucosyltransferase activity capable of modifying oligomannosidic N-glycans was detected in axenic Dictyostelium extracts. The presence of fucose on the N-glycans and the reactivity to the antiserum, but not the fucosyltransferase activity, are abolished in the fucose-deficient HL250 strain. In later stages of development, N-glycans at the mound and culmination stages show a reduction in both the size and the degree of modification by intersecting/bisecting residues compared with mid-exponential phase cultures, consistent with the hypothesis that glycosidase and glycosyltransferase expression levels are altered during the slime mould life cycle.

Author information

Author/s: Schiller, Birgit (B); Hykollari, Alba (A); Voglmeir, Josef (J); Pöltl, Gerald (G); Hummel, Karin (K); Razzazi-Fazeli, Ebrahim (E); Geyer, Rudolf (R); Wilson, Iain B H (IB);

Affiliation: Department für Chemie, Universität für Bodenkultur, Wien, Austria.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Biochemical journal (Biochem J), published in England. (Language: eng)

Reference: 2009-Oct; vol 423 (issue 1) : pp 41-52

Dates: Created 2009/09/10; Completed 2009/10/05;

PMID: 19614564, status: MEDLINE (last retrieval date: 10/5/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence Animals Carbohydrate Dehydrogenases - genetics; metabolism Carbohydrate Sequence Cells, Cultured Dictyostelium - genetics; growth & development; metabolism; ultrastructure

Dictyostelium - genetics; growth & development; metabolism; ultrastructure Fucose - chemistry; metabolism Fucosyltransferases - metabolism Germ-Free Life - genetics; physiology Glycosylation Polysaccharides - chemistry; metabolism

Associated Chemicals: Polysaccharides (0) ; Fucose (3713-31-3) ; Carbohydrate Dehydrogenases (EC 1.1.-) ; GDPmannose dehydrogenase (EC 1.1.1.132) ; Fucosyltransferases (EC 2.4.1.-)

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