Research article summary (published 30 Oct 2009):

Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells.

Full Abstract

BACKGROUND: Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARbeta) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS: In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARbeta and PDLIM4 promoters. Forced Myc overexpression inhibited the RARbeta and PDLIM4 expression. RESULTS: Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-2'-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARbeta and PDLIM4. CONCLUSION: The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b.

Author information

Author/s: He, Meilan (M); Vanaja, Donkena Krishna (DK); Karnes, R Jeffrey (RJ); Young, Charles Y F (CY);

Affiliation: Department of Urology, Mayo Clinic, Rochester, Minnesota.

Grants: CA 91956 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: The Prostate (Prostate), published in United States. (Language: eng)

Reference: 2009-Nov; vol 69 (issue 15) : pp 1643-50

Dates: Created 2009/09/28; Completed 2009/10/15;

PMID: 19623543, status: MEDLINE (last retrieval date: 10/15/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Blotting, Western Cell Growth Processes - genetics Cell Line DNA - genetics DNA (Cytosine-5-)-Methyltransferase - metabolism DNA Methylation DNA-Binding Proteins - genetics Epigenesis, Genetic Gene Expression Profiling

Genes, myc Humans Male Polymerase Chain Reaction Prostate - enzymology; physiology Prostatic Neoplasms - genetics; pathology Receptors, Retinoic Acid - genetics Sequence Analysis, DNA Transfection

Associated Chemicals: DNA-Binding Proteins (0) ; PDLIM4 protein, human (0) ; Receptors, Retinoic Acid (0) ; retinoic acid receptor beta (0) ; DNA (9007-49-2) ; DNA methyltransferase 3B (EC 2.1.1.-) ; DNA (Cytosine-5-)-Methyltransferase (EC 2.1.1.37) ; DNA methyltransferase 3A (EC 2.1.1.37)

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