Research article summary (published 22 Jul 2009):

Involvement of extracellular ascorbate and iron in hydroxyl radical generation in rat striatum in carbon monoxide poisoning.

Full Abstract

Carbon monoxide (CO) poisoning stimulated generation in rat striatum of toxic hydroxyl radicals (*OH), which might participate in the CO-induced neuronal injury. Since an increase in extracellular ascorbate (AA) stimulated *OH generation in the presence of endogenous metals, including iron, in rat striatum in vivo, we examined the role of extracellular AA in *OH generation due to CO poisoning in the present study. The CO-induced *OH generation in the striatum was strongly suppressed by intrastriatal administration of active, but not inactivated, AA oxidase, which degrades extracellular AA. In addition, CO poisoning caused a significant increase in extracellular AA in rat striatum, suggesting a role of extracellular AA in the CO-induced *OH generation. However, the time-course of changes in extracellular AA could not be completely superimposed on that of the CO-induced *OH generation. On the other hand, the CO-induced *OH generation was completely suppressed by an iron chelator, deferoxamine. These findings suggest that *OH generation in rat striatum due to CO poisoning may involve both extracellular AA and chelatable iron. 2009 Elsevier Ireland Ltd.

Author information

Author/s: Hara, Shuichi (S); Mizukami, Hajime (H); Mukai, Toshiji (T); Kurosaki, Kunihiko (K); Kuriiwa, Fumi (F); Endo, Takahiko (T);

Affiliation: Department of Forensic Medicine, Tokyo Medical University, Tokyo 160-8402, Japan. s-hara(-atsign-)tokyo-med.ac.jp

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Toxicology (Toxicology), published in Ireland. (Language: eng)

Reference: 2009-Oct; vol 264 (issue 1-2) : pp 69-73

Dates: Created 2009/09/07; Completed 2009/09/23;

PMID: 19632292, status: MEDLINE (last retrieval date: 9/23/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Ammonia - metabolism Animals Antioxidants Ascorbate Oxidase - pharmacology Ascorbic Acid - metabolism; physiology Brain Chemistry - drug effects Carbon Monoxide Poisoning - metabolism Catechols - pharmacology Hydroxyl Radical - metabolism

Hydroxyl Radical - metabolism Iron - metabolism; physiology Iron Chelating Agents - pharmacology Male Microdialysis Neostriatum - drug effects; metabolism Rats Rats, Sprague-Dawley Stereotaxic Techniques

Associated Chemicals: 2,3-dihydroxy benzoic acid (0) ; Antioxidants (0) ; Catechols (0) ; Iron Chelating Agents (0) ; Hydroxyl Radical (3352-57-6) ; Ascorbic Acid (50-81-7) ; Iron (7439-89-6) ; Ammonia (7664-41-7) ; Ascorbate Oxidase (EC 1.10.3.3)

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