Research article summary (published 22 Jul 2009):

Macrolide resistance in microorganisms at antimicrobial-free Swine farms.

Full Abstract

To investigate the relationship between agricultural antimicrobial use and resistance, a variety of methods for quantification of macrolide-lincosamide-streptogramin B (MLS(B)) resistance were applied to organic swine farm manure samples. Fluorescence in situ hybridization was used to indirectly quantify the specific rRNA methylation resulting in MLS(B) resistance. Using this method, an unexpectedly high prevalence of ribosomal methylation and, hence, predicted MLS(B) resistance was observed in manure samples from two swine finisher farms that reported no antimicrobial use (37.6% +/- 6.3% and 40.5% +/- 5.4%, respectively). A culture-based method targeting relatively abundant clostridia showed a lower but still unexpectedly high prevalence of resistance at both farms (27.7% +/- 11.3% and 11.7% +/- 8.6%, respectively), while the prevalence of resistance in cultured fecal streptococci was low at both farms (4.0%). These differences in the prevalence of resistance across microorganisms suggest the need for caution when extrapolating from data obtained with indicator organisms. A third antimicrobial-free swine farm, a breeder-to-finisher operation, had low levels of MLS(B) resistance in manure samples with all methods used (<9%). Tetracycline antimicrobials were detected in manure samples from one of the finisher farms and may provide a partial explanation for the high level of MLS(B) resistance. Taken together, these findings highlight the need for a more fundamental understanding of the relationship between antimicrobial use and the prevalence of antimicrobial resistance.

Author information

Author/s: Zhou, Zhi (Z); Raskin, Lutgarde (L); Zilles, Julie L (JL);

Affiliation: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Applied and environmental microbiology (Appl Environ Microbiol), published in United States. (Language: eng)

Reference: 2009-Sep; vol 75 (issue 18) : pp 5814-20

Dates: Created 2009/09/09; Completed 2009/11/02;

PMID: 19633121, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Anti-Bacterial Agents - pharmacology Drug Resistance, Bacterial Drug Utilization - statistics & numerical data Feces - chemistry; microbiology Gram-Positive Bacteria - drug effects; isolation & purification In Situ Hybridization, Fluorescence - methods

Lincosamides - pharmacology Macrolides - pharmacology Methylation RNA, Ribosomal - genetics; metabolism Streptogramin B - pharmacology Swine - microbiology Tetracyclines - analysis

Associated Chemicals: Anti-Bacterial Agents (0) ; Lincosamides (0) ; Macrolides (0) ; RNA, Ribosomal (0) ; Tetracyclines (0) ; Streptogramin B (3131-03-1)

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