In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus.

Full Abstract

Vibrio vulnificus is a Gram-negative bacterium that induces severely rapid pathological progress. In this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, COS A (MW, 10,000 Da) and COS B (MW, 1,000 Da), from 90-95% deacetylated chitosan, against V. vulnificus in vitro and in vivo. Treatment with COS A resulted in significantly higher suppressive effects on the growth of V. vulnificus than treatment with COS B. The growth of V. vulnificus was inhibited within 1 h of treatment with water-soluble COS A in concentrations ranging from 0.5 to 10 mg/ml. Additionally, treatment with COS A completely inhibited V. vulnificus-induced cytotoxicity in human intestinal epithelial INT-407 cells, while COS B did not. Furthermore, the administration of COS A (0.1-0.5 mg per mouse) significantly increased the survival period of V. vulnificus-infected mice. The number of viable V. vulnificus in the spleen, liver, small intestine, and blood was significantly lower in COS A-treated mice than in untreated mice. Here, we clearly demonstrate that COS A is a potential agent for the prevention and treatment of infection with V. vulnificus.

Author information

Author/s: Lee, Byung Cheol (BC); Kim, Myun Soo (MS); Choi, Sang Ho (SH); Kim, Kwang Yoon (KY); Kim, Tae Sung (TS);

Affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of molecular medicine (Int J Mol Med), published in Greece. (Language: eng)

Reference: 2009-Sep; vol 24 (issue 3) : pp 327-33

Dates: Created 2009/07/29; Completed 2009/09/30;

PMID: 19639224, status: MEDLINE (last retrieval date: 9/30/2009, IMS Date: )

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