[Research of extracting and purifying taxol from the branches of Taxus media]

Full Abstract

OBJECTIVE: The study was to explore extracting and purifying technology of taxol from the branches of Taxus media. METHOD: In extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC. RESULT: The results showed that the recovery of extracting toxal with the method of ultrasonic was the highest and the selectivity of supercritical CO2 fluid extraction was the best; The sample after being extracted should be purified two stages by silica gel column chromatography with dichloromethane-chloroform-methane (53:44:3) as elution and the lamella chromatography was chloroform-ethyl acetate-methane (88:7:5) as elution, finally we reached the fawn crystal. CONCLUSION: In that production, the content of taxol reached 87. 3% and the recovery 89.7%, which indicat that the taxol is well enrichment.

Author information

Author/s: Qiao, Liangjie (L); Man, Ruilin (R); Ni, Wangdong (W); Liang, Yonghuang (Y);

Affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi), published in China. (Language: chi)

Reference: 2009-Apr; vol 34 (issue 8) : pp 973-6

Dates: Created 2009/07/30; Completed 2009/10/09;

PMID: 19639778, status: MEDLINE (last retrieval date: 10/9/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.