Research article summary (published 30 Oct 2009):

A novel androgen-dependent prostate cancer xenograft model derived from skin metastasis of a Japanese patient.

Full Abstract

BACKGROUND: The incidence of, and mortality from, prostate cancer (PCa) has increased in Asian countries over the past decades, partly due to a change in dietary habits. Recent reports have revealed differences in the molecular basis of PCa among people of differing racial or ethnic backgrounds. PCa xenograft models established from Asian patients would be useful for understanding the basis of PCa in Asian populations; we therefore established and characterized a novel PCa xenograft model, JDCaP, from a metastatic skin lesion of a Japanese hormone-refractory prostate cancer (HRPC) patient. METHODS: Skin metastatic tissue derived from poorly differentiated prostatic adenocarcinoma in a 61-year-old Japanese male was transplanted to nude mice and JDCaP was established by serial passage. Expression of androgen receptor (AR) and prostate-specific antigen (PSA) was evaluated by immunohistochemistry and the AR sequence was analyzed. Hormone sensitivity of JDCaP was investigated in vivo by orchiectomy followed by administration of steroid hormones, including testosterone, estradiol, progesterone, and hydrocortisone. Therapeutic effects of leuprorelin acetate, bicalutamide, flutamide, diethylstilbestrol (DES), and estradiol were investigated. RESULTS: JDCaP expressed wild-type AR and PSA and showed androgen dependence. Only testosterone administration maintained tumor proliferation after orchiectomy. Administration of leuprorelin acetate, bicalutamide, and flutamide inhibited tumor growth. DES and estradiol also demonstrated significant antitumor effects. CONCLUSIONS: JDCaP expresses wild-type ARs and exhibits androgen dependence despite its origin from a HRPC patient. The model may be useful to elucidate the molecular basis of PCa in Asian populations and to develop prevention and therapeutic strategies.

Author information

Author/s: Kimura, Takahiro (T); Kiyota, Hiroshi (H); Nakata, Daisuke (D); Masaki, Tsuneo (T); Kusaka, Masami (M); Egawa, Shin (S);

Affiliation: Department of Urology, Jikei University School of Medicine, Tokyo, Japan. tkimura(-atsign-)jikei.ac.jp

Journal and publication information

Publication Type: Journal Article

Journal: The Prostate (Prostate), published in United States. (Language: eng)

Reference: 2009-Nov; vol 69 (issue 15) : pp 1660-7

Dates: Created 2009/09/28; Completed 2009/10/15;

PMID: 19644954, status: MEDLINE (last retrieval date: 10/15/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenocarcinoma - metabolism; secondary Animals Antigens, Neoplasm - metabolism Antineoplastic Agents, Hormonal - pharmacology Asian Continental Ancestry Group Diethylstilbestrol - pharmacology Humans Leuprolide - pharmacology Male Mice

Mice, Inbred BALB C Mice, Nude Middle Aged Neoplasm Transplantation Neoplasms, Hormone-Dependent - metabolism; pathology Prostatic Neoplasms - metabolism; pathology Receptors, Androgen - metabolism Skin Neoplasms - metabolism; secondary Specific Pathogen-Free Organisms Transplantation, Heterologous

Associated Chemicals: Antigens, Neoplasm (0) ; Antineoplastic Agents, Hormonal (0) ; Receptors, Androgen (0) ; prostate cancer antigen 3, human (0) ; Leuprolide (53714-56-0) ; Diethylstilbestrol (56-53-1)

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