A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling.

Full Abstract

Fluorescent proteins (FPs) with far-red excitation and emission are desirable for multicolor labeling and live-animal imaging. We describe E2-Crimson, a far-red derivative of the tetrameric FP DsRed-Express2. Unlike other far-red FPs, E2-Crimson is noncytotoxic in bacterial and mammalian cells. E2-Crimson is brighter than other far-red FPs and matures substantially faster than other red and far-red FPs. Approximately 40% of the E2-Crimson fluorescence signal is remarkably photostable. With an excitation maximum at 611 nm, E2-Crimson is the first FP that is efficiently excited with standard far-red lasers. We show that E2-Crimson has unique applications for flow cytometry and stimulated emission depletion (STED) microscopy.

Author information

Author/s: Strack, Rita L (RL); Hein, Birka (B); Bhattacharyya, Dibyendu (D); Hell, Stefan W (SW); Keenan, Robert J (RJ); Glick, Benjamin S (BS);

Affiliation: Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

Grants: R01 EB008087 (Agency:NIBIB NIH HHS) ; T32 BM007183 (Agency:FDA HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Biochemistry (Biochemistry), published in United States. (Language: eng)

Reference: 2009-Sep; vol 48 (issue 35) : pp 8279-81

Dates: Created 2009/09/01; Completed 2009/09/25;

PMID: 19658435, status: MEDLINE (last retrieval date: 9/25/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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