Detection of lfrA and tap efflux pump genes among clinical isolates of non-pigmented rapidly growing mycobacteria.

Full Abstract

This study was performed to detect LfrA and Tap efflux pumps among clinical isolates of non-pigmented rapidly growing mycobacteria (NPRGM). Gene detection was performed using polymerase chain reaction (PCR) with specific primers designed for each gene. Susceptibility of the strains to doxycycline, tigecycline and ciprofloxacin was analysed using the broth microdilution reference technique. In total, 166 clinical isolates were included in the study. The lfrA gene was detected in four strains (2.4%), comprising two strains of Mycobacterium chelonae (6.7% of this species), one Mycobacterium fortuitum (1.1%) and one Mycobacterium mucogenicum (14.3%). The tap gene was detected in 109 strains (65.7%), comprising 3 Mycobacterium abscessus (33.3%), 12 M. chelonae (40%), 75 M. fortuitum (84.3%), 2 Mycobacterium mageritense (40%), 15 Mycobacterium peregrinum (68.2%), 1 Mycobacterium alvei and 1 Mycobacterium porcinum; no strains of M. mucogenicum were tap-positive. No differences between tap-positive and -negative strains were observed for resistance to doxycycline (Fisher's exact test, P=0.055). lfrA is rare among clinical isolates of NPRGM, whilst tap is found more commonly. No correlation was detected between the presence of the efflux pumps and resistance to quinolones or tetracyclines.

Author information

Author/s: Esteban, J (J); Martín-de-Hijas, N Z (NZ); Ortiz, A (A); Kinnari, T J (TJ); Bodas Sánchez, A (A); Gadea, I (I); Fernández-Roblas, R (R);

Affiliation: Department of Clinical Microbiology, Fundación Jiménez Díaz-UTE, Av. Reyes Católicos 2, 28040 Madrid, Spain. jestebanmoreno(-atsign-)gmail.com

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of antimicrobial agents (Int J Antimicrob Agents), published in Netherlands. (Language: eng)

Reference: 2009-Nov; vol 34 (issue 5) : pp 454-6

Dates: Created 2009/09/15; Completed 2009/11/06;

PMID: 19665358, status: MEDLINE (last retrieved date: 11/6/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Anti-Bacterial Agents - pharmacology
Antiporters - genetics
Bacterial Proteins - genetics
Ciprofloxacin - pharmacology
DNA Primers - genetics
DNA, Bacterial - genetics
Doxycycline - pharmacology
Drug Resistance, Multiple, Bacterial

Humans
Membrane Transport Proteins - genetics
Microbial Sensitivity Tests
Minocycline - analogs & derivatives; pharmacology
Mycobacterium - genetics; isolation & purification
Mycobacterium Infections - microbiology
Polymerase Chain Reaction - methods
Spain

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Anti-Bacterial Agents (0) ; Antiporters (0) ; Bacterial Proteins (0) ; DNA Primers (0) ; DNA, Bacterial (0) ; Membrane Transport Proteins (0) ; Tap protein, Mycobacterium (0) ; tigecycline (0) ; Minocycline (10118-90-8) ; Doxycycline (564-25-0) ; Ciprofloxacin (85721-33-1)

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