Research article summary (published 6 Aug 2009):

An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene.

Full Abstract

Lecithin:retinol acyltransferase (LRAT) is essential for vitamin A storage. Nuclear run-on assays demonstrated transcriptional regulation of the Lrat gene in vivo by all-trans-retinoic acid (RA) and other retinoids. Analysis of a 2.5 kb segment of rat genomic DNA revealed that the region approximately 300 bp upstream from the transcription start site (TSS) is necessary for high luciferase (Luc) reporter activity in HEK293T and HepG2 cells. Although this region lacks retinoid receptor binding elements, it responded to the nuclear receptors RARalpha, RARbeta or RARgamma, with RXRalpha, with and without ligand. Removal of -111 bp from the TSS, which is well conserved in human, rat and mouse genomes, completely eliminated activity. This region contains several basic elements (TATA box, SP3 site, AP-1 site, CAAT box), all of which were essential. Nuclear extracts from RA-treated cells exhibited enhanced binding. Therefore, this proximal region together with basal transcription factors may be sufficient to drive Lrat expression.

Author information

Author/s: Zolfaghari, Reza (R); Ross, A Catharine (AC);

Affiliation: Department of Nutritional Sciences, Pennsylvania State University, University Park, PA 16802, USA.

Grants: CA-90214 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys), published in United States. (Language: eng)

Reference: 2009-Sep; vol 489 (issue 1-2) : pp 1-9

Dates: Created 2009/09/14; Completed 2009/09/29; Revised 2009/10/05;

PMID: 19665987, status: MEDLINE (last retrieved date: 10/6/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Acyltransferases - biosynthesis; genetics Animals Base Sequence - genetics Cell Line, Tumor Gene Expression Regulation, Enzymologic - physiology Genome, Human - physiology Humans Mice

Mice Rats Receptors, Retinoic Acid - genetics; metabolism Response Elements - physiology Sequence Deletion - genetics TATA Box - physiology Transcription, Genetic - physiology Vitamin A - genetics; metabolism

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Receptors, Retinoic Acid (0) ; Vitamin A (11103-57-4) ; Acyltransferases (EC 2.3.-) ; lecithin-retinol acyltransferase (EC 2.3.1.-)

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