Research article summary (published 13 Nov 2009):

Carbonic anhydrase IX and human papillomavirus as diagnostic biomarkers of cervical dysplasia/neoplasia in women with a cytologic diagnosis of atypical glandular cells: a Gynecologic Oncology Group study in United States.

Full Abstract

High-risk human papillomavirus (H-HPV) infection is strongly linked to cervical neoplasia, but its role in detecting glandular lesions (GLs) is unclear. In the cervix, carbonic anhydrase IX (CA-IX) is expressed in cervical neoplasia, but rarely in the benign cervix. The diagnostic utility of these biomarkers was evaluated in women with a cytologic diagnosis of atypical glandular cells (AGC). H-HPV was detected using hybrid capture 2 (HC2) in liquid-based cytology, and CA-IX immunoreactivity was studied on conventional Pap smears. Of 403 patients, 111 (28%) were positive for significant cervical lesions (SCLs) including CIN2, CIN3, adenocarcinoma in situ or invasive carcinoma. CA-IX testing alone (n = 403) had a sensitivity of 75, 95 or 65% for SCLs, significant GLs or squamous lesions (SLs), respectively, with a specificity of 88% and a false negative rate (FNR defined as 1 minus negative predictive value) of 10%. Testing for H-HPV (n = 122) had a sensitivity of 97, 100 or 96% for SCLs, GLs or SLs, respectively, with a specificity of 87% and a FNR of 1%. The combination of CA-IX and H-HPV testing (n = 122), collectively, had the same sensitivity, specificity and FNR for SCLs, GLs or SLs as H-HPV testing alone. The conclusions of our study are that both H-HPV and CA-IX testing are useful diagnostic markers for GLs. However, H-HPV testing is a better diagnostic marker for SLs. The combination of CA-IX with H-HPV testing does not improve the diagnostic accuracy for cervical neoplasia in women with AGC diagnosis over that of H-HPV testing alone.

Author information

Author/s: Liao, Shu-Yuan (SY); Rodgers, William H (WH); Kauderer, James (J); Bonfiglio, Thomas A (TA); Walker, Joan L (JL); Darcy, Kathleen M (KM); Carter, Randy (R); Hatae, Masayuji (M); Levine, Lyuba (L); Spirtos, Nick M (NM); Stanbridge, Eric J (EJ);

Affiliation: Department of Epidemiology, University of California at Irvine, School of Medicine, Irvine, CA.

Grants: CA 11479 (Agency:NCI NIH HHS) ; CA 27469 (Agency:NCI NIH HHS) ; CA 37517 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Nov; vol 125 (issue 10) : pp 2434-40

Dates: Created 2009/09/28; Completed 2009/11/05;

PMID: 19670419, status: MEDLINE (last retrieval date: 11/5/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenocarcinoma - diagnosis; enzymology; virology Adult Aged Aged, 80 and over Antigens, Neoplasm - metabolism Carbonic Anhydrases - metabolism Carcinoma, Squamous Cell - diagnosis; enzymology; virology Cervical Intraepithelial Neoplasia - diagnosis; enzymology; virology Cytodiagnosis DNA, Viral - genetics Female Genotype Humans Immunoenzyme Techniques

Middle Aged Neoplasm Invasiveness Neoplasm Staging Neoplasms, Glandular and Epithelial - diagnosis; enzymology; virology Papillomaviridae - genetics; isolation & purification Papillomavirus Infections - diagnosis; enzymology; virology Polymerase Chain Reaction Retrospective Studies Risk Factors Sensitivity and Specificity Tumor Markers, Biological - genetics; metabolism Uterine Cervical Neoplasms - diagnosis; enzymology; virology Vaginal Smears Young Adult

Associated Chemicals: Antigens, Neoplasm (0) ; DNA, Viral (0) ; Tumor Markers, Biological (0) ; CA9 protein, human (EC 4.2.1.1) ; Carbonic Anhydrases (EC 4.2.1.1)

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