Research article summary (published 29 Nov 2009):

Cell cycle-related kinase supports ovarian carcinoma cell proliferation via regulation of cyclin D1 and is a predictor of outcome in patients with ovarian carcinoma.

Full Abstract

Our previous study has suggested that the cell cycle-related kinase (CCRK) is a putative candidate oncogene in glioblastoma tumorigenesis. The potential oncogenic role of CCRK and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, CCRK expression was examined by immunohistochemistry in a series of ovarian carcinoma tissues. Overexpression of CCRK was detected in 53% of the ovarian carcinomas, and it was positively correlated with an ascending histological grade and/or advanced clinical stage of the disease (p < 0.05). In addition, overexpression of CCRK in ovarian carcinoma was determined to be a strong and an independent predictor of short overall survival (p < 0.05). In ovarian carcinoma cells, CCRK knockdown by RNAi led to a G1 phase cell cycle arrest, while CCRK overexpression by stable transfection of CCRK-containing plasmid pcDNA-CCRK promoted cell proliferation in vitro and tumor growth in vivo. In addition, CCRK knockdown was found to reduce cyclin D1 expression. Consistently, CCRK overexpression increased cyclin D1 expression, and furthermore, a significant correlation between expression of CCRK and cyclin D1 in ovarian carcinomas was observed (p < 0.001). These findings suggest a potential important role of CCRK in the control of cell proliferation via regulation of cyclin D1 expression, and the overexpression of CCRK, as detected by immunohistochemistry, is an independent molecular marker for shortened survival time of patients with ovarian carcinoma.

Author information

Author/s: Wu, Guo-Qing (GQ); Xie, Dan (D); Yang, Guo-Feng (GF); Liao, Yi-Ji (YJ); Mai, Shi-Juan (SJ); Deng, Hai-Xia (HX); Sze, Johnny (J); Guan, Xin-Yuan (XY); Zeng, Yi-Xin (YX); Lin, Marie C (MC); Kung, Hsiang-Fu (HF);

Affiliation: State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Dec; vol 125 (issue 11) : pp 2631-42

Dates: Created 2009/10/05; Completed 2009/11/06;

PMID: 19672860, status: MEDLINE (last retrieval date: 11/6/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Aged Animals Apoptosis Blotting, Western Case-Control Studies Cell Proliferation Cohort Studies Cyclin D1 - metabolism Cyclin-Dependent Kinases - antagonists & inhibitors; genetics; metabolism Cystadenocarcinoma, Serous - genetics; metabolism; secondary Female Gene Expression Regulation, Neoplastic Humans

Immunoenzyme Techniques Mice Mice, Inbred BALB C Middle Aged Ovarian Neoplasms - genetics; metabolism; pathology Ovary - metabolism; pathology Phosphorylation Prognosis RNA, Messenger - genetics; metabolism RNA, Small Interfering - pharmacology Reverse Transcriptase Polymerase Chain Reaction Survival Rate Tissue Array Analysis Tumor Cells, Cultured

Associated Chemicals: CCND1 protein, human (0) ; RNA, Messenger (0) ; RNA, Small Interfering (0) ; Cyclin D1 (136601-57-5) ; Cyclin-Dependent Kinases (EC 2.7.1.37) ; cyclin-dependent kinase-activating kinase (EC 2.7.1.37)

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