Layer II/III of the prefrontal cortex: Inhibition by the serotonin 5-HT1A receptor in development and stress.

Full Abstract

The modulation of the prefrontal cortex by the neurotransmitter serotonin (5-HT) is thought to play a key role in determining adult anxiety levels. Layer II/III of the prefrontal cortex, which mediates communication across cortical regions, displays a high level of 5-HT(1A) receptor binding in normal individuals and a significantly lower level in patients with mood and anxiety disorders. Here, we examine how serotonin modulates pyramidal neurons in layer II/III of the rat prefrontal cortex throughout postnatal development and in adulthood. Using whole cell recordings in brain slices of the rat medial prefrontal cortex, we observed that serotonin directly inhibits layer II/III pyramidal neurons through 5-HT(1A) receptors across postnatal development (postnatal days 6-96). In adulthood, a sex difference in these currents emerges, consistent with human imaging studies of 5-HT(1A) receptor binding. We examined the effects of early life stress on the 5-HT(1A) receptor currents in layer II/III. Surprisingly, animals subjected to early life stress displayed significantly larger 5-HT(1A)-mediated outward currents throughout the third and fourth postnatal weeks after elevated 5-HT(1A) expression during the second postnatal week. Subsequent exposure to social isolation in adulthood resulted in the almost-complete elimination of 5-HT(1A) currents in layer II/III neurons suggesting an interaction between early life events and adult experiences. These data represent the first examination of functional 5-HT(1A) receptors in layer II/III of the prefrontal cortex during normal development as well as after stress.

Author information

Author/s: Goodfellow, Nathalie M (NM); Benekareddy, Madhurima (M); Vaidya, Vidita A (VA); Lambe, Evelyn K (EK);

Affiliation: Department of Physiology, University of Toronto, Ontario, Canada.

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci), published in United States. (Language: eng)

Reference: 2009-Aug; vol 29 (issue 32) : pp 10094-103

Dates: Created 2009/08/13; Completed 2009/09/08; Revised 2009/10/23;

PMID: 19675243, status: MEDLINE (last retrieval date: 10/26/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.