IL-33 activates unprimed murine basophils directly in vitro and induces their in vivo expansion indirectly by promoting hematopoietic growth factor production.

Full Abstract

IL-33, a new member of the IL-1 family, has been described as an important inducer of Th2 cytokines and mediator of inflammatory responses. In this study, we demonstrate that murine basophils sorted directly from the bone marrow, without prior exposure to IL-3 or Fc(epsilon)R cross-linking, respond to IL-33 alone by producing substantial amounts of histamine, IL-4, and IL-6. These cells express ST2 constitutively and generate a cytokine profile that differs from their IL-3-induced counterpart by a preferential production of IL-6. In vivo, IL-33 promotes basophil expansion in the bone marrow (BM) through an indirect mechanism of action depending on signaling through the beta(c) chain shared by receptors for IL-3, GM-CSF, and IL-5. IL-3 can still signal through its specific beta(IL-3) chain in these mutant mice, which implies that it is not the unique growth-promoting mediator in this setup, but requires IL-5 and/or GMCSF. Our results support a major role of the latter growth factor, which is readily generated by total BM cells as well as sorted basophils in response to IL-33 along with low amounts of IL-3. Furthermore, GM-CSF amplifies IL-3-induced differentiation of basophils from BM cells, whereas IL-5 that is also generated in vivo, affects neither their functions nor their growth in vitro or in vivo. In conclusion, our data provide the first evidence that IL-33 not only activates unprimed basophils directly, but also promotes their expansion in vivo through induction of GM-CSF and IL-3.

Author information

Author/s: Schneider, Elke (E); Petit-Bertron, Anne-France (AF); Bricard, Rachel (R); Levasseur, Mélanie (M); Ramadan, Abdelrauf (A); Girard, Jean-Philippe (JP); Herbelin, André (A); Dy, Michel (M);

Affiliation: Université Paris Descartes, Faculté de Médecine-Centre National de la Recherche Scientifique Unité Mixte de Recherche 8147, Paris, France. schneider(-atsign-)necker.fr

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol), published in United States. (Language: eng)

Reference: 2009-Sep; vol 183 (issue 6) : pp 3591-7

Dates: Created 2009/09/03; Completed 2009/09/23;

PMID: 19684081, status: MEDLINE (last retrieval date: 9/23/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Basophils - cytology
Bone Marrow Cells - cytology
Cell Proliferation
Colony-Stimulating Factors - biosynthesis
Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis

Associated Chemicals: Colony-Stimulating Factors (0) ; Interleukin-3 (0) ; Interleukin-5 (0) ; Interleukins (0) ; interleukin-33, mouse (0) ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)

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