Research article summary (published 29 Sep 2009):

Expression pattern alterations of the serine protease HtrA1 in normal human placental tissues and in gestational trophoblastic diseases.

Full Abstract

HtrA1 is a secreted protein which behaves as a molecular chaperone at low temperatures and as a serine protease at high temperatures. When the placenta escapes the normal growth control mechanisms, which are present during normal pregnancy, it may develop trophoblastic diseases, such as hydatidiform mole and choriocarcinoma. The aim of the study is to investigate the expression of HtrA1 in these gestational trophoblastic diseases and evaluate whether different HtrA1 expression might be associated with increasingly severe forms of disease. We used immunohistochemistry to assess the expression of HtrA1 in normal human placenta, hydatidiform mole (partial and complete) and choriocarcinoma. In addition to that we used the western blotting technique to quantify HtrA1 immunoreaction in normal human placentas. The most striking finding of our investigation is the decrease in immunostaining of this protease with increasing severity of gestational trophoblastic disease. For instance, in partial and complete moles HtrA1 is weakly expressed in the trophoblast. Moreover, absence of immunoreaction for HtrA1 is observable in the choriocarcinoma cells. In conclusion, we suggest that HtrA1 may play an important role in the pathogenesis and progression of hydatidiform moles and choriocarcinomas, and that HtrA1 may play an important role during the normal development of the placenta, as well as in trophoblastic diseases.

Author information

Author/s: Marzioni, Daniela (D); Quaranta, Alexia (A); Lorenzi, Teresa (T); Morroni, Manrico (M); Crescimanno, Caterina (C); De Nictolis, Michele (M); Toti, Paolo (P); Muzzonigro, Giovanni (G); Baldi, Alfonso (A); De Luca, Antonio (A); Castellucci, Mario (M);

Affiliation: Department of Molecular Pathology and Innovative Therapies, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. d.marzioni(-atsign-)univpm.it

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Histology and histopathology (Histol Histopathol), published in Spain. (Language: eng)

Reference: 2009-Oct; vol 24 (issue 10) : pp 1213-22

Dates: Created 2009/08/18; Completed 2009/11/03;

PMID: 19688690, status: MEDLINE (last retrieval date: 11/3/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence Case-Control Studies Choriocarcinoma - metabolism; pathology Female Gestational Age Gestational Trophoblastic Neoplasms - metabolism; pathology Glutathione Transferase - metabolism Humans

Humans Hydatidiform Mole - metabolism; pathology Immunohistochemistry Molecular Sequence Data Placenta - chemistry; metabolism; pathology Pregnancy Recombinant Fusion Proteins - chemistry; metabolism Serine Endopeptidases - chemistry; genetics; metabolism

Associated Chemicals: Recombinant Fusion Proteins (0) ; Glutathione Transferase (EC 2.5.1.18) ; HtrA1 protein, human (EC 3.4.21.-) ; Serine Endopeptidases (EC 3.4.21.-)

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