Research article summary (published 16 Aug 2009):

DNA synthesis of rat bone marrow mesenchymal stem cells through alpha1-adrenergic receptors.

Full Abstract

Multipotential bone marrow mesenchymal stem cells (BMSCs) are important in maintaining the microenvironment of the bone marrow (BM). Sympathetic nerves histologically innervate the BM; however, their role remains unclear. In this study, the effects of norepinephrine on DNA synthesis and the related signaling molecules involved in rBMSCs were examined. mRNA levels of the alpha1-adrenergic receptor subtypes increased following norepinephrine stimulation (10(-5) M for 30 min). DNA synthesis increased in dose- and time-dependent manners as determined by [(3)H]thymidine incorporation. Intracellular Ca(2+) concentration and translocation of protein kinase C from the cytosol to the membrane were also found to be elevated in rBMSCs. Phentolamine was able to suppress translocation of PKC. Norepinephrine also induced phosphorylation of ERK1/2, which was prevented by staurosporine treatment. Pretreatment with PD98059 inhibited ERK1/2 phosphorylation and DNA synthesis in rBMSCs. These findings indicate that norepinephrine stimulates DNA synthesis via alpha1-adrenergic receptors and downstream Ca(2+)/PKC and ERK1/2 activation in rBMSCs.

Author information

Author/s: Han, Jing (J); Zou, Zhongmin (Z); Zhu, Caizhong (C); Deng, Jun (J); Wang, Junping (J); Ran, Xinze (X); Shi, Chunmeng (C); Ai, Guoping (G); Li, Rong (R); Cheng, Tianmin (T); Su, Yongping (Y);

Affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys), published in United States. (Language: eng)

Reference: 2009-Oct; vol 490 (issue 2) : pp 96-102

Dates: Created 2009/10/13; Completed 2009/10/30;

PMID: 19695215, status: MEDLINE (last retrieval date: 10/30/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adipocytes - cytology; metabolism Animals Base Sequence Bone Marrow - innervation Calcium Signaling - drug effects Cell Differentiation Cell Proliferation - drug effects Cells, Cultured DNA - biosynthesis DNA Primers - genetics Flavonoids - pharmacology Hematopoietic Stem Cells - drug effects; metabolism

Hematopoietic Stem Cells - drug effects; metabolism Mesenchymal Stem Cells - drug effects; metabolism Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism Multipotent Stem Cells - drug effects; metabolism Norepinephrine - pharmacology Osteoblasts - cytology; metabolism Phentolamine - pharmacology Protein Kinase C - metabolism RNA, Messenger - genetics; metabolism Rats Receptors, Adrenergic, alpha-1 - genetics; metabolism

Associated Chemicals: DNA Primers (0) ; Flavonoids (0) ; PD 98059 (0) ; RNA, Messenger (0) ; Receptors, Adrenergic, alpha-1 (0) ; Phentolamine (50-60-2) ; Norepinephrine (51-41-2) ; DNA (9007-49-2) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.1.37) ; Mitogen-Activated Protein Kinase 3 (EC 2.7.1.37) ; Protein Kinase C (EC 2.7.11.13)

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