Research article summary (published 30 Jul 2009):

Magnetic tagging increases delivery of circulating progenitors in vascular injury.

Full Abstract

OBJECTIVES: We sought to magnetically tag endothelial progenitor cells (EPCs) with a clinical agent and target them to a site of arterial injury using a magnetic device positioned outside the body. BACKGROUND: Circulating EPCs are involved in physiological processes such as vascular re-endothelialization and post-ischemic neovascularization. However, the success of cell therapies depends on the ability to deliver the cells to the site of injury. METHODS: Human EPCs were labeled with iron oxide superparamagnetic nanoparticles. Cell viability and differentiation were tested using flow cytometry. Following finite element modeling computer simulations and flow testing in vitro, angioplasty was performed on rat common carotid arteries to denude the endothelium and EPCs were administered with and without the presence of an external magnetic device for 12 min. RESULTS: Computer simulations indicated successful external magnetic cell targeting from a vessel with flow rate similar to a rat common carotid artery; correspondingly there was a 6-fold increase in cell capture in an in vitro flow system. Targeting enhanced cell retention at the site of injury by 5-fold at 24 h after implantation in vivo. CONCLUSIONS: Using an externally applied magnetic device, we have been able to enhance EPC localization at a site of common carotid artery injury. This technology could be more widely adapted to localize cells in other organs and may provide a useful tool for the systemic injection of cell therapies.

Author information

Author/s: Kyrtatos, Panagiotis G (PG); Lehtolainen, Pauliina (P); Junemann-Ramirez, Manfred (M); Garcia-Prieto, Ana (A); Price, Anthony N (AN); Martin, John F (JF); Gadian, David G (DG); Pankhurst, Quentin A (QA); Lythgoe, Mark F (MF);

Affiliation: Centre for Advanced Biomedical Imaging, University College London Department of Medicine and UCL Institute of Child Health, London, United Kingdom.

Grants: (Agency:Biotechnology and Biological Sciences Research Council) ; (Agency:British Heart Foundation)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: JACC. Cardiovascular interventions (JACC Cardiovasc Interv), published in United States. (Language: eng)

Reference: 2009-Aug; vol 2 (issue 8) : pp 794-802

Dates: Created 2009/08/21; Completed 2009/10/29;

PMID: 19695550, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

CommentIn: JACC Cardiovasc Interv. 2009 Aug;2(8):803-4. (PMID: 19695551)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Antigens, CD - analysis Apoptosis Carotid Artery Injuries - pathology; surgery Cell Differentiation Cell Movement Cell Survival Cells, Cultured Computer Simulation Disease Models, Animal Endothelial Cells - immunology; transplantation Feasibility Studies Ferrosoferric Oxide Finite Element Analysis Flow Cytometry

Glycoproteins - analysis Humans Magnetics Male Microscopy, Confocal Models, Cardiovascular Nanoparticles Peptides - analysis Pilot Projects Rats Rats, Sprague-Dawley Staining and Labeling - methods Stem Cell Transplantation - methods Stem Cells - immunology Time Factors

Associated Chemicals: AC133 antigen (0) ; Antigens, CD (0) ; Glycoproteins (0) ; Peptides (0) ; ferumoxides (119683-68-0) ; Ferrosoferric Oxide (1317-61-9)

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