Research article summary (published 24 Aug 2009):

DNA-based MRI probes for specific detection of chronic exposure to amphetamine in living brains.

Full Abstract

We designed phosphorothioate-modified DNA probes linked to superparamagnetic iron oxide nanoparticles (SPION) for in vivo magnetic resonance imaging (MRI) of fosB and Delta fosB mRNA after amphetamine (AMPH) exposure in mice. Specificity of both the fosB and Delta fosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obtained from acutely AMPH-exposed mouse brains. We confirmed time-dependent uptake and retention profiles of both probes in neurons of GAD67-green fluorescent protein knock-in mice. MRI signal of SPION-labeled fosB probe delivered via intracerebroventricular route was elevated in both acutely and chronically AMPH-exposed mice; the signal was suppressed by dopaminergic receptor antagonist pretreatment. SPION-labeled Delta fosB probe signal elevation occurred only in chronically AMPH-exposed mice. The in vivo target specificity of these probes permits reliable MRI visualization of AMPH-induced differential elevations of fosB and Delta fosB mRNA in living brains.

Author information

Author/s: Liu, Christina H (CH); Ren, Jia Q (JQ); Yang, Jinsheng (J); Liu, Charng-ming (CM); Mandeville, Joseph B (JB); Rosen, Bruce R (BR); Bhide, Pradeep G (PG); Yanagawa, Yuchio (Y); Liu, Philip K (PK);

Affiliation: Laboratory for Gene Transcript Targeting, Imaging and Repair, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.

Grants: P30 NS045776-06A17807 (Agency:NINDS NIH HHS) ; P30NS045776 (Agency:NINDS NIH HHS) ; P41 RR014075-10 (Agency:NCRR NIH HHS) ; P41RR014075 (Agency:NCRR NIH HHS) ; R01 DA020796-03 (Agency:NIDA NIH HHS) ; R01 DA026108-01 (Agency:NIDA NIH HHS) ; R01 EB002066-19 (Agency:NIBIB NIH HHS) ; R01 NS045845-04 (Agency:NINDS NIH HHS) ; R01DA020796 (Agency:NIDA NIH HHS) ; R01DA026108 (Agency:NIDA NIH HHS) ; R01EB002066 (Agency:NIBIB NIH HHS) ; R01NS045845 (Agency:NINDS NIH HHS) ; R03EB008134 (Agency:NIBIB NIH HHS) ; R21 DA024235-02 (Agency:NIDA NIH HHS) ; R21 NS057556-01A1S1 (Agency:NINDS NIH HHS) ; R21 NS057556-02 (Agency:NINDS NIH HHS) ; R21DA024235 (Agency:NIDA NIH HHS) ; R21NS057556 (Agency:NINDS NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci), published in United States. (Language: eng)

Reference: 2009-Aug; vol 29 (issue 34) : pp 10663-70

Dates: Created 2009/08/27; Completed 2009/09/10; Revised 2009/09/21;

PMID: 19710318, status: MEDLINE (last retrieval date: 9/22/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amphetamine - pharmacology Animals Brain - drug effects; metabolism Central Nervous System Stimulants - pharmacology Contrast Media - metabolism DNA - metabolism Drug Administration Schedule Gene Expression Regulation - drug effects Glutamate Decarboxylase - genetics; metabolism Green Fluorescent Proteins Injections, Intraventricular - methods

Injections, Intraventricular - methods Magnetic Resonance Imaging - methods Male Metal Nanoparticles Mice Mice, Inbred C57BL Mice, Transgenic Phosphorothioate Oligonucleotides - metabolism Proto-Oncogene Proteins c-fos - genetics; metabolism RNA, Messenger - metabolism Time Factors

Associated Chemicals: Central Nervous System Stimulants (0) ; Contrast Media (0) ; Fosb protein, mouse (0) ; Phosphorothioate Oligonucleotides (0) ; Proto-Oncogene Proteins c-fos (0) ; RNA, Messenger (0) ; Green Fluorescent Proteins (147336-22-9) ; Amphetamine (300-62-9) ; DNA (9007-49-2) ; Glutamate Decarboxylase (EC 4.1.1.15) ; glutamate decarboxylase 1 (EC 4.1.1.15)

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