Research article summary (published 30 Aug 2009):

Type II collagen levels correlate with mineralization by articular cartilage vesicles.

Full Abstract

OBJECTIVE: Pathologic mineralization is common in osteoarthritic (OA) cartilage and may be mediated by extracellular organelles known as articular cartilage vesicles (ACVs). Paradoxically, ACVs isolated from OA human cartilage mineralize poorly in vitro compared with those isolated from normal porcine cartilage. We recently showed that collagens regulate ACV mineralization. We sought to determine differences between collagens and collagen receptors on human and porcine ACVs as a potential explanation of their different mineralization behaviors. METHODS: ACVs were enzymatically released from old and young human and porcine hyaline articular cartilage. Western blotting was used to determine the presence of types I, II, VI, and X collagen and various collagen receptors on ACVs. Type II collagen was quantified by enzyme-linked immunosorbent assay. Biomineralization was assessed by measuring the uptake of (45)Ca by isolated ACVs in agarose gels and by ACVs in situ in freeze-thawed cartilage. RESULTS: As previously shown, isolated human ACVs mineralized poorly in response to ATP compared with porcine ACVs, but human and porcine ACVs mineralized similarly in situ in freeze-thawed cartilage. Type II collagen levels were 100-fold higher in isolated human ACVs than in porcine ACVs. Type II collagen in human ACVs was of high molecular weight. Transglutaminase-crosslinked type II collagen showed increased resistance to collagenase, suggesting a possible explanation for residual collagen on human ACVs. Expression of other collagens and collagen receptors was similar on human and porcine ACVs. CONCLUSION: Higher levels of type II collagen in human ACV preparations, perhaps mediated by increased transglutaminase crosslinking, may contribute to the decreased mineralization observed in isolated human ACVs in vitro.

Author information

Author/s: Jubeck, Brian (B); Muth, Emily (E); Gohr, Claudia M (CM); Rosenthal, Ann K (AK);

Affiliation: Rheumatology Section, Medical College of Wisconsin and Zablocki VA Medical Center, 5000 West National Avenue, Milwaukee, WI 53295, USA.

Grants: R01 AR 056215 (Agency:NIAMS NIH HHS) ; R01 AR052615-03 (Agency:NIAMS NIH HHS)

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural

Journal: Arthritis and rheumatism (Arthritis Rheum), published in United States. (Language: eng)

Reference: 2009-Sep; vol 60 (issue 9) : pp 2741-6

Dates: Created 2009/09/21; Completed 2009/10/19;

PMID: 19714645, status: MEDLINE (last retrieval date: 10/19/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adolescent Adult Aged Aged, 80 and over Animals Calcium Phosphates - metabolism Calcium Pyrophosphate - metabolism Cartilage, Articular - metabolism; pathology

Collagen Type II - metabolism Humans Organelles - metabolism; pathology Osteoarthritis - metabolism; pathology Receptors, Collagen - metabolism Swine Transglutaminases - metabolism Young Adult

Associated Chemicals: Calcium Phosphates (0) ; Collagen Type II (0) ; Receptors, Collagen (0) ; Calcium Pyrophosphate (10086-45-0) ; calcium phosphate (10103-46-5) ; Transglutaminases (EC 2.3.2.13)

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