Research article summary (published 29 Sep 2009):

Determination of nivalenol and deoxynivalenol by liquid chromatography/atmospheric pressure photoionization mass spectrometry.

Full Abstract

Fusarium species, a plant pathogenic fungus of wheat and other cereals, produces toxic metabolites such as nivalenol (NIV) and deoxynivalenol (DON). Control of contamination by these toxins is very difficult, and a continuous survey of the occurrence is necessary for these toxins. Thus, the accurate and convenient determination of the cereals contaminated with these toxins is important for the supply of safe foods. A selective analytical method based on high-performance liquid chromatography, combined with atmospheric pressure photoionization (APPI) mass spectrometry, has been developed for simultaneous determination of NIV and DON. The parameters investigated for the optimization of APPI were the ion source parameters fragmentor voltage, capillary voltage, and vaporizer temperature, and also mobile phase composition and flow rate. Furthermore, chemical noise and signal suppression of analyte signals due to sample matrix interference were investigated for APPI. The results indicated that APPI provides lower matrix effect and the correlation coefficient of NIV and DON in the range 0.2-100 ng x mL(-1) was above 0.999. Recoveries of NIV and DON in wheat ranged from 86 to 107% and limits of detection of NIV and DON were 0.20 ng x g(-1) and 0.39 ng x g(-1), respectively. In addition, the proposed method was applied for the analysis of naturally contaminated wheat samples. APPI was found to offer lower matrix effect and was a convenient technique for routine analysis of NIV and DON residues in wheat at trace levels. Copyright (c) 2009 John Wiley & Sons, Ltd.

Author information

Author/s: Tanaka, Hiroki (H); Takino, Masahiko (M); Sugita-Konishi, Yoshiko (Y); Tanaka, Toshitsugu (T); Toriba, Akira (A); Hayakawa, Kazuichi (K);

Affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. hiroki.t(-atsign-)poem.ocn.ne.jp

Journal and publication information

Publication Type: Evaluation Studies; Journal Article

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2009-Oct; vol 23 (issue 19) : pp 3119-24

Dates: Created 2009/09/08; Completed 2009/10/27;

PMID: 19714706, status: MEDLINE (last retrieval date: 10/27/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Chromatography, High Pressure Liquid - methods Mass Spectrometry - methods Mycotoxins - analysis

Mycotoxins - analysis Trichothecenes - analysis Triticum - chemistry

Associated Chemicals: Mycotoxins (0) ; Trichothecenes (0) ; nivalenol (23282-20-4) ; deoxynivalenol (51481-10-8)

Related articles

These are the highest related articles currently in the database:

• Determination of nivalenol and deoxynivalenol in wheat using liquid chromatography-mass spectrometry with negative ion atmospheric pressure chemical ionisation. 25 Aug 1999
• Fate of deoxynivalenol in contaminated wheat grain during preparation of Egyptian 'balila'. 29 Apr 2007
• Determination of deoxynivalenol in white flour, whole wheat flour, and bran by solid-phase extraction/liquid chromatography: interlaboratory study. 29 Jun 1998
• Determination of trichothecene mycotoxins in wheat by use of supercritical fluid extraction and high-performance liquid chromatography with diode array detection or gas chromatography with electron capture detection. 4 Feb 1998
• Analysis of deoxynivalenol, masked deoxynivalenol, and Fusarium graminearum pigment in wheat samples, using liquid chromatography-UV-mass spectrometry. 30 May 2008
• Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry. 2 May 2005
• Validation of an HPLC analytical method coupled to a multifunctional clean-up column for the determination of deoxynivalenol. 30 Mar 2006
• A rapid multiresidual determination of type A and type B trichothecenes in wheat flour by HPLC-ESI-MS. 27 Feb 2005
• Rapid simultaneous determination of major type A- and B-trichothecenes as well as zearalenone in maize by high performance liquid chromatography-tandem mass spectrometry. 12 Jan 2005
• Simultaneous quantification of A-trichothecene mycotoxins in grains using liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry. 28 Aug 2002

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.