Presynaptic CaV2 calcium channel traffic requires CALF-1 and the alpha(2)delta subunit UNC-36.

Full Abstract

Presynaptic voltage-gated calcium channels provide calcium for synaptic vesicle exocytosis. We show here that a green fluorescent protein-tagged alpha(1) subunit of the Caenorhabditis elegans CaV2 channel, UNC-2, is localized to presynaptic active zones of sensory and motor neurons. Synaptic localization of CaV2 requires the alpha(2)delta subunit UNC-36 and CALF-1 (Calcium Channel Localization Factor-1), a neuronal transmembrane protein that localizes to the endoplasmic reticulum. In calf-1 mutants, UNC-2 is retained in the endoplasmic reticulum, but other active-zone components and synaptic vesicles are delivered to synapses. Acute induction of calf-1 mobilizes preexisting UNC-2 for delivery to synapses, consistent with a direct trafficking role. The alpha(2)delta subunit UNC-36 is likewise required for exit of UNC-2 from endoplasmic reticulum but has additional functions. Genetic and cell biological interactions suggest that CALF-1 couples intracellular traffic to functional maturation of CaV2 presynaptic calcium channels.

Author information

Author/s: Saheki, Yasunori (Y); Bargmann, Cornelia I (CI);

Affiliation: Howard Hughes Medical Institute and Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, New York, USA.

Grants: (Agency:Howard Hughes Medical Institute)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Nature neuroscience (Nat Neurosci), published in United States. (Language: eng)

Reference: 2009-Oct; vol 12 (issue 10) : pp 1257-65

Dates: Created 2009/09/28; Completed 2009/10/13;

PMID: 19718034, status: MEDLINE (last retrieval date: 10/13/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Animals, Genetically Modified
Behavior, Animal
Caenorhabditis elegans
Caenorhabditis elegans Proteins - genetics; metabolism; physiology
Calcium - metabolism
Calcium Channels - metabolism
Chi-Square Distribution
Chromosome Mapping - methods
Endoplasmic Reticulum - metabolism
Epithelial Cells - metabolism; ultrastructure
Exocytosis
Gene Expression Regulation - genetics; physiology

Gene Expression Regulation - genetics; physiology
Green Fluorescent Proteins - genetics
Intestines - cytology
Membrane Proteins - genetics; metabolism; physiology
Mutation
Nerve Tissue Proteins - genetics; metabolism
Neurons - cytology; metabolism; ultrastructure
Presynaptic Terminals - metabolism
Protein Subunits - metabolism
Protein Transport - genetics; physiology
Swimming - physiology
Synapses - physiology
Vacuolar Proton-Translocating ATPases - physiology

Associated Chemicals: Caenorhabditis elegans Proteins (0) ; Calcium Channels (0) ; Membrane Proteins (0) ; Nerve Tissue Proteins (0) ; Protein Subunits (0) ; calcium channel cocalization factor-1, C elegans (0) ; unc-2 protein, C elegans (0) ; Green Fluorescent Proteins (147336-22-9) ; Calcium (7440-70-2) ; Vacuolar Proton-Translocating ATPases (EC 3.6.1.-) ; unc-32 protein, C elegans (EC 3.6.1.-)

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