Toxoplasma gondii: a simple Real-time PCR assay to quantify the proliferation of the apicoplast.

Full Abstract

A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicoplast DNA copies in the test samples. The results indicated that the copies of T. gondii apicoplast DNA was significantly low when exposed to ciprofloxacin, clindamycin and azithromycin for 48h in the second infectious cycle. Our study shows that the Real-time PCR technique is a simple and quick technique for screening the anti-apicoplast drugs.

Author information

Author/s: Wu, Liang (L); Chen, Sheng-Xia (SX); Jiang, Xu-Gan (XG); Cao, Jian-Ping (JP);

Affiliation: School of Medical Technology, Jiangsu University, Zhenjiang, People's Republic of China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Experimental parasitology (Exp Parasitol), published in United States. (Language: eng)

Reference: 2009-Dec; vol 123 (issue 4) : pp 384-7

Dates: Created 2009/10/20; Completed 2009/10/27;

PMID: 19720060, status: MEDLINE (last retrieval date: 10/27/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Anti-Infective Agents - pharmacology
Azithromycin - pharmacology
Ciprofloxacin - pharmacology
Clindamycin - pharmacology
Computer Systems
DNA, Protozoan - analysis

DNA, Protozoan - analysis
Electrophoresis, Agar Gel
Hela Cells
Humans
Plastids - drug effects; genetics
Polymerase Chain Reaction - methods
Toxoplasma - drug effects; genetics; ultrastructure

Associated Chemicals: Anti-Infective Agents (0) ; DNA, Protozoan (0) ; Clindamycin (18323-44-9) ; Azithromycin (83905-01-5) ; Ciprofloxacin (85721-33-1)

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