Research article summary (published 29 Sep 2009):

Combination treatment of hydrogen peroxide and X-rays induces apoptosis in human prostate cancer PC-3 cells.

Full Abstract

PURPOSE: To study the effect of hydrogen peroxide (H(2)O(2)) on radiation-induced apoptosis in human prostate cancer PC-3 cells. METHODS AND MATERIALS: At 4h before the irradiation, PC-3 cells were exposed to 10mM ammonium chloride (NH(4)Cl) concentrations. Subsequently, cells were exposed to 0.1mM H(2)O(2) just before the irradiations, which were administered with 10-MV X-rays at doses of 10 Gy. RESULTS: The percentage of apoptotic cells at 48 h after X-irradiation alone, H(2)O(2) alone, and combined X-irradiation and H(2)O(2) was 1.85%, 4.85%, and 28.4%, respectively. With use of combined X-irradiation and H(2)O(2), production of reactive oxygen species (ROS) occurred 4h after the irradiation. This resulted in lysosomal rupturing, mitochondrial fragmentation, and the release of cytochrome c into the cytoplasm from the mitochondria. In contrast, when cells were exposed to NH(4)Cl before the X-irradiation and H(2)O(2) administration, apoptosis was almost completely suppressed, ROS production did not occur, lysosomal rupture and mitochondrial fragmentation were blocked, and cytochrome c was not released. CONCLUSIONS: Hydrogen peroxide strongly enhanced lysosome-dependent radiation-induced apoptosis in human prostate cancer PC-3 cells. A combined use of X-rays and H(2)O(2) can also injure the mitochondrial cytoplasmic organelles and lead to the production of ROS that in and of itself might possibly induce apoptosis.

Author information

Author/s: Kariya, Shinji (S); Sawada, Ken (K); Kobayashi, Toshihiro (T); Karashima, Takashi (T); Shuin, Taro (T); Nishioka, Akihito (A); Ogawa, Yasuhiro (Y);

Affiliation: Department of Diagnostic Radiology and Radiation Oncology, Kochi Medical School, Kochi, Japan. kariyas(-atsign-)kochi-u.ac.jp

Journal and publication information

Publication Type: Journal Article

Journal: International journal of radiation oncology, biology, physics (Int J Radiat Oncol Biol Phys), published in United States. (Language: eng)

Reference: 2009-Oct; vol 75 (issue 2) : pp 449-54

Dates: Created 2009/09/08; Completed 2009/09/21;

PMID: 19735867, status: MEDLINE (last retrieval date: 9/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Ammonium Chloride - pharmacology Apoptosis - drug effects; radiation effects Cell Line, Tumor - drug effects; radiation effects Cytochromes c - metabolism Humans Hydrogen Peroxide - pharmacology Lysosomes - drug effects; radiation effects Male

Male Mitochondria - drug effects; radiation effects Organelles - drug effects; radiation effects Oxidants - pharmacology Oxidative Stress Prostatic Neoplasms - metabolism; pathology Radiotherapy Dosage Reactive Oxygen Species - metabolism

Associated Chemicals: Oxidants (0) ; Reactive Oxygen Species (0) ; Ammonium Chloride (12125-02-9) ; Hydrogen Peroxide (7722-84-1) ; Cytochromes c (9007-43-6)

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