Characterization of leptospires using V3 region of 16S rDNA by denaturing gradient gel electrophoresis: a case study.

Full Abstract

Serological and molecular characterization of Leptospiral isolates helps us to identify serovar, which is useful, for epidemiological study. Serological characterization is tedious and requires a panel of monoclonal antibodies and expertise to read the results. This study is a preliminary work to evaluate the usefulness of Denaturing Gradient Gel Electrophoresis (DGGE) to identify serovars of leptospira. The V3 region of most conserved 16S rDNA of five pathogenic leptospiral serovars and one saprophytic serovar was characterized. DGGE method was employed to separate the amplified V3 region based on the nucleotide sequence. On DGGE, amplified V3 region of leptospiral serovars, under study, showed bands at different positions indicating DGGE as the effective method of characterization in the future. DNA sequencing of V3 region of the three serovars showed great difference in nucleotide sequence supporting the results of DGGE.

Author information

Author/s: Pol, S S (SS); Dhakephalkar, P K (PK); Bharadwaj, R S (RS);

Affiliation: Department of Microbiology, B.J. Medical College, Sassoon Hospital Campus, Station Road, Pune-411 001, India. aparnapol(-atsign-)rediffmail.com

Journal and publication information

Publication Type: Evaluation Studies; Journal Article

Journal: Indian journal of medical microbiology (Indian J Med Microbiol), published in India. (Language: eng)

Reference: -2009 Oct-Dec; vol 27 (issue 4) : pp 354-7

Dates: Created 2009/09/08; Completed 2009/11/02;

PMID: 19736407, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.