Effects of salivary gland homogenate from wild-caught and laboratory-reared Lutzomyia longipalpis on the evolution and immunomodulation of Leishmania (Leishmania) amazonensis infection.

Full Abstract

We investigated the effects of Lutzomyia longipalpis salivary glands homogenate of wild-caught and laboratory-reared vectors on the lesion evolution and immunomodulation of the infection caused by Leishmania (Leishmania) amazonensis. To compare the effect of both salivary glands homogenate (SGH), C57BL/6 mice were inoculated subcutaneously into the hind footpads or into the ear dermis with 10(6) promastigotes in the presence or not of SGH from wild-caught and laboratory-colonized sand flies. Comparing SGH groups, the lesion size was lower in mice co-inoculated with wild-caught SGH, as the parasitism and the infiltration of macrophages at the inoculation site. Wild-caught SGH also determined lower production of IL-4 and IL-10 but higher IL-12 levels compared with laboratory-reared SGH. Our findings address a probable bias by using SGH from laboratory-colonized sand flies instead of wild-caught vector SGH in studies concerning saliva effects. A possible mild influence of sand fly saliva in natural infections caused by Leishmania is also speculated, as infection is transmitted by wild and not by laboratory-reared vectors.

Author information

Author/s: Laurenti, M D (MD); da Matta, V L R (VL); Pernichelli, T (T); Secundino, N F C (NF); Pinto, L C (LC); Corbett, C E P (CE); Pimenta, P P F (PP);

Affiliation: Laboratory of Pathology of Infectious Diseases (LIM-50), Department of Pathology, Medical School, University of São Paulo, São Paulo, SP, Brazil. mdlauren(-atsign-)usp.br

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Scandinavian journal of immunology (Scand J Immunol), published in England. (Language: eng)

Reference: 2009-Oct; vol 70 (issue 4) : pp 389-95

Dates: Created 2009/09/15; Completed 2009/10/12;

PMID: 19751274, status: MEDLINE (last retrieval date: 10/12/2009, IMS Date: )

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