Research article summary (published 29 Sep 2009):

Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield.

Full Abstract

Generating a new variety of plant with erect-leaf is a critical strategy to improve rice grain yield, as plants with this trait can be dense-planted. The erect-leaf is a significant morphological trait partially regulated by Brassinosteroids (BRs) in rice plants. So far, only a few genes can be used for molecular breeding in rice. Here, we identified OsBAK1 as a potential gene to alter rice architecture. Based on rice genome sequences, four closely related homologs of Arabidopsis BAK1 (AtBAK1) gene were amplified. Phylogenetic analysis and suppression of a weak Arabidopsis mutant bri1-5 indicated that OsBAK1 (Os08g0174700) is the closest relative of AtBAK1. Genetic, physiological, and biochemical analyses all suggest that the function of OsBAK1 is conserved with AtBAK1. Overexpression of a truncated intracellular domain of OsBAK1, but not the extracellular domain of OsBAK1, resulted in a dwarfed phenotype, similar to the rice BR-insensitive mutant plants. The expression of OsBAK1 changed important agricultural traits of rice such as plant height, leaf erectness, grain morphologic features, and disease resistance responses. Our results suggested that a new rice variety with erect-leaf and normal reproduction can be generated simply by suppressing the expression level of OsBAK1. Therefore, OsBAK1 is a potential molecular breeding tool for improving rice grain yield by modifying rice architecture.

Author information

Author/s: Li, Dan (D); Wang, Lei (L); Wang, Min (M); Xu, Yun-Yuan (YY); Luo, Wei (W); Liu, Ya-Ju (YJ); Xu, Zhi-Hong (ZH); Li, Jia (J); Chong, Kang (K);

Affiliation: Research Center for Molecular & Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Plant biotechnology journal (Plant Biotechnol J), published in England. (Language: eng)

Reference: 2009-Oct; vol 7 (issue 8) : pp 791-806

Dates: Created 2009/09/16; Completed 2009/10/21;

PMID: 19754838, status: MEDLINE (last retrieval date: 10/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Arabidopsis - genetics; metabolism Arabidopsis Proteins - genetics Breeding Gene Expression Regulation, Plant Oryza sativa - genetics; metabolism Phylogeny

Plant Growth Regulators - metabolism Plant Leaves - genetics; metabolism Plant Proteins - genetics; metabolism Plants, Genetically Modified - genetics; metabolism Protein-Serine-Threonine Kinases - genetics RNA, Plant - genetics

Associated Chemicals: Arabidopsis Proteins (0) ; Plant Growth Regulators (0) ; Plant Proteins (0) ; RNA, Plant (0) ; BAK1 protein, Arabidopsis (EC 2.7.1.-) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1)

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