An innovative triple immunogold labeling method to investigate the hemopoietic stem cell niche in situ.

Full Abstract

The ultrastructural study of rare cells within their niche in situ is very difficult. We have developed a method for locating individual transplanted cells and simultaneously identifying and analyzing the molecules and cellular phenotypes surrounding them in situ using transmission electron microscopy. This innovative method involves triple immunogold labeling combined with serial ultrathin sectioning. We demonstrate the validity of this approach by examining the niche of individual transplanted cells from a population highly enriched for hemopoietic stem cells and the ultrastructural expression of two key stem cell regulatory molecules, hyaluronic acid and osteopontin. In addition, we describe the phenotypes of the surrounding cells.

Author information

Author/s: Ellis, Sarah L (SL); Williams, Brenda (B); Asquith, Stephen (S); Bertoncello, Ivan (I); Nilsson, Susan K (SK);

Affiliation: Microscopy Core Facility, Peter MacCallum Cancer Centre, East Melbourne, Victoria 3002, Australia.

Journal and publication information

Publication Type: Journal Article

Journal: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (Microsc Microanal), published in United States. (Language: eng)

Reference: 2009-Oct; vol 15 (issue 5) : pp 403-14

Dates: Created 2009/09/16; Completed 2009/11/06;

PMID: 19754979, status: MEDLINE (last retrieval date: 11/6/2009, IMS Date: )

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