Automated assay for fondaparinux (Arixtra) on the Dade Behring BCS XP.

Full Abstract

An assay for fondaparinux (Arixtra) is described based on a modified commercial assay for heparin. The assay is automated on a Dade Behring BCS XP (Siemens Healthcare Diagnostics, Deerfield, IL) and uses the inhibition of activated factor X to quantitate the drug. The assay was unaffected by platelet contamination or the presence of warfarin. The assay was affected by the antithrombin level, and the value obtained in the assay decreased significantly when the antithrombin level was less than 60%. The assay is, however, not specific for fondaparinux. Specimens containing unfractionated or low-molecular-weight heparin will yield results by this assay that will not be an accurate estimation of concentration.

Author information

Author/s: Sanfelippo, Michael J (MJ); Tillema, Veronica B (VB);

Affiliation: Department of Hematology/Coagulation Services, Marshfield Labs, Marshfield Clinic, Marshfield, WI 54449, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: American journal of clinical pathology (Am J Clin Pathol), published in United States. (Language: eng)

Reference: 2009-Oct; vol 132 (issue 4) : pp 608-12

Dates: Created 2009/09/18; Completed 2009/10/01;

PMID: 19762539, status: MEDLINE (last retrieval date: 10/1/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Associated Chemicals: Antithrombins (0) ; Heparin, Low-Molecular-Weight (0) ; Polysaccharides (0) ; fondaparinux (0) ; Heparin (9005-49-6) ; Factor Xa (EC 3.4.21.6)

These are the highest related articles currently in the database: