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| Research article summary (published 15 Sep 2009): |
Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs).
Full Abstract
The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically and biophysically distinct. Here we use well-defined in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs in vivo.
Author information
Author/s: Seib, F Philipp (FP); Prewitz, Marina (M); Werner, Carsten (C); Bornhäuser, Martin (M);
Affiliation: Leibniz Institute for Polymer Research, Max Bergmann Centre for Biomaterials Dresden, Hohe Strasse 6, Dresden, Germany.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)
Reference: 2009-Nov; vol 389 (issue 4) : pp 663-7
Dates: Created 2009/10/14; Completed 2009/11/02;
PMID: 19766096, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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