Research article summary (published 21 Sep 2009):

A secretory phospholipase A2-mediated neuroprotection and anti-apoptosis.

Full Abstract

BACKGROUND: Phospholipase A2 liberates free fatty acids and lysophospholipids upon hydrolysis of phospholipids and these products are often associated with detrimental effects such as inflammation and cerebral ischemia. The neuroprotective effect of neutral phospholipase from snake venom has been investigated. RESULTS: A neutral anticoagulant secretory phospholipase A2 (nPLA) from the venom of Naja sputatrix (Malayan spitting cobra) has been found to reduce infarct volume in rats subjected to focal transient cerebral ischemia and to alleviate the neuronal damage in organotypic hippocampal slices subjected to oxygen-glucose deprivation (OGD). Real-time PCR based gene expression analysis showed that anti-apoptotic and pro-survival genes have been up-regulated in both in vivo and in vitro models. Staurosporine or OGD mediated apoptotic cell death in astrocytoma cells has also been found to be reduced by nPLA with a corresponding reduction in caspase 3 activity. CONCLUSION: We have found that a secretory phospholipase (nPLA) purified from snake venom could reduce infarct volume in rodent stroke model. nPLA, has also been found to reduce neuronal cell death, apoptosis and promote cell survival in vitro ischemic conditions. In all conditions, the protective effects could be seen at sub-lethal concentrations of the protein.

Author information

Author/s: Armugam, Arunmozhiarasi (A); Cher, Charmian D N (CD); Lim, KaiYing (K); Koh, Dawn C I (DC); Howells, David W (DW); Jeyaseelan, Kandiah (K);

Affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. bchaa(-atsign-)nus.edu.sg

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: BMC neuroscience (BMC Neurosci), published in England. (Language: eng)

Reference: 2009-; vol 10 (issue ) : pp 120

Dates: Created 2009/10/07; Completed 2009/10/12;

PMID: 19775433, status: MEDLINE (last retrieved date: 10/12/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Analysis of Variance Animals Anoxia Apoptosis - drug effects Brain - drug effects; pathology Brain Ischemia - drug therapy; metabolism; pathology Cell Survival - drug effects Chromatography, High Pressure Liquid Cobra Venoms - chemistry Glucose - deficiency Hippocampus - drug effects; metabolism; pathology Infarction, Middle Cerebral Artery - drug therapy; metabolism; pathology

Infarction, Middle Cerebral Artery - drug therapy; metabolism; pathology Male Microarray Analysis Neurons - drug effects; metabolism Neuroprotective Agents - therapeutic use Organ Culture Techniques Phospholipases A2 - isolation & purification; therapeutic use Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Tissue Plasminogen Activator - metabolism Tumor Cells, Cultured

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Cobra Venoms (0) ; Neuroprotective Agents (0) ; Glucose (50-99-7) ; Phospholipases A2 (EC 3.1.1.4) ; Tissue Plasminogen Activator (EC 3.4.21.68)

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