Very low-molecular-mass fragments of albumin in the plasma of patients with focal segmental glomerulosclerosis.

Full Abstract

BACKGROUND: Primary focal segmental glomerulosclerosis (FSGS) is a glomerular disease that frequently does not respond to treatment and progresses to kidney failure. FSGS can be of either genetic origin, caused by mutations in slit diaphragm proteins, such as podocin, or idiopathic origin of unknown cause. STUDY DESIGN: Case series. SETTING & PARTICIPANTS: Children with FSGS (aged 3-18 years); 15 with idiopathic and 11 with genetic forms of FSGS. PREDICTOR: Genetic versus idiopathic forms. OUTCOMES & MEASUREMENTS: Differentially expressed proteins in the plasma proteome, detected using 2-dimensional electrophoresis and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Western blot, and liquid chromatography electron spray ionization tandem mass spectrometry for fragmentation and identification of the peptides. RESULTS: We found 3 very low-molecular-mass (9.2, 6.9, and 4.7 kDa; isoelectric point, 5.7) spots that were present in pooled samples from patients with genetic FSGS, but missing in patients with idiopathic FSGS and healthy individuals. Spots were identified using mass spectrometry as fragments of albumin, 2 of them apparently containing peptides from both C- and N-terminal parts of the whole protein. Proteomic analyses were carried out on all genetic patients individually; of these, 10 of 11 patients had > or =1 albumin fragment detected in the pool. We did not find an evident relationship between type of mutation or clinical status of patients and albumin fragments observed. LIMITATIONS: Very low-molecular-weight albumin fragments also can be produced by other diseases. CONCLUSIONS: We describe for the first time the presence of very low-molecular-mass albumin fragments in plasma of patients with FSGS with podocyte protein mutations that are absent in patients with idiopathic FSGS or healthy individuals. Additional studies are necessary to determine whether these fragments could be potential biomarkers to distinguish between genetic and idiopathic forms of FSGS.

Author information

Author/s: Hellin, Joan Lopez (JL); Bech-Serra, Joan J (JJ); Moctezuma, Enrique Lara (EL); Chocron, Sara (S); Santin, Sheila (S); Madrid, Alvaro (A); Vilalta, Ramon (R); Canals, Francesc (F); Torra, Roser (R); Meseguer, Anna (A); Nieto, Jose L (JL);

Affiliation: Fisopatologia Renal, CIBBIM, Institut de Recerca, Hospital Vall d'Hebron, Barcelona, Spain. joalopez(-atsign-)ir.vhebron.net

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: American journal of kidney diseases : the official journal of the National Kidney Foundation (Am J Kidney Dis), published in United States. (Language: eng)

Reference: 2009-Nov; vol 54 (issue 5) : pp 871-80

Dates: Created 2009/10/26; Completed 2009/11/16;

PMID: 19781833, status: MEDLINE (last retrieved date: 11/16/2009)

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