Runx2, osx, and dspp in tooth development.

Full Abstract

The transcription factors Runx2 and Osx are necessary for osteoblast and odontoblast differentiation, while Dspp is important for odontoblast differentiation. The relationship among Runx2, Osx, and Dspp during tooth and craniofacial bone development remains unknown. In this study, we hypothesized that the roles of Runx2 and Osx in the regulation of osteoblast and odontoblast lineages may be independent of one another. The results showed that Runx2 expression overlapped with Osx in dental and osteogenic mesenchyme from E12 to E16. At the later stages, from E18 to PN14, Runx2 and Osx expressions remained intense in alveolar bone osteoblasts. However, Runx2 expression was down-regulated, whereas Osx expression was clearly seen in odontoblasts. At later stages, Dspp transcription was weakly present in osteoblasts, but strong in odontoblasts where Osx was highly expressed. In mouse odontoblast-like cells, Osx overexpression increased Dspp transcription. Analysis of these data suggests differential biological functions of Runx2, Osx, and Dspp during odontogenesis and osteogenesis.

Author information

Author/s: Chen, S (S); Gluhak-Heinrich, J (J); Wang, Y H (YH); Wu, Y M (YM); Chuang, H H (HH); Chen, L (L); Yuan, G H (GH); Dong, J (J); Gay, I (I); MacDougall, M (M);

Affiliation: Department of Pediatric Dentistry, TheUniversity of Texas Health Science Center at San Antonio,7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. Chens0(-atsign-)uthscsa.edu

Grants: DE014484 (Agency:NIDCR NIH HHS) ; DE113221 (Agency:NIDCR NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal: Journal of dental research (J Dent Res), published in United States. (Language: eng)

Reference: 2009-Oct; vol 88 (issue 10) : pp 904-9

Dates: Created 2009/09/28; Completed 2009/11/02;

PMID: 19783797, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alveolar Process - cytology
Ameloblasts - cytology; physiology
Animals
Cell Differentiation - physiology
Cell Line, Tumor
Cell Lineage - physiology
Cells, Cultured
Core Binding Factor Alpha 1 Subunit - analysis; physiology
Dental Pulp - cytology
Mesoderm - cytology; physiology
Mice

Mice
Mice, Inbred ICR
Odontoblasts - cytology; physiology
Odontogenesis - physiology
Osteoblasts - cytology; physiology
Osteogenesis - physiology
Phosphoproteins - analysis; physiology
Protein Precursors - analysis; physiology
Sialoglycoproteins - analysis; physiology
Transcription Factors - analysis; physiology
Zinc Fingers - physiology

Associated Chemicals: Core Binding Factor Alpha 1 Subunit (0) ; Dspp protein, mouse (0) ; Phosphoproteins (0) ; Protein Precursors (0) ; Runx2 protein, mouse (0) ; Sialoglycoproteins (0) ; Transcription Factors (0) ; osterix protein, mouse (0)

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