Research article summary (published 29 Sep 2009):

Comparison of preservation solutions in an experimental model of organ cooling in kidney transplantation.

Full Abstract

BACKGROUND: Viscous preservation solutions such as University of Wisconsin solution (UW) may be less effective at rapid removal of blood from an organ so that cooling takes longer. This study assessed the temperature changes of kidneys flushed with UW and hyperosmolar citrate (HOC). METHODS: Porcine kidneys were retrieved and flushed with 500 ml UW or HOC at 4 degrees C while monitoring kidney temperature at depths of 5 and 20 mm. Renal function was measured on an isolated organ preservation system. RESULTS: The mean(s.d.) rate of temperature fall was slower with UW (at 20 mm: 0.64(0.11) versus 1.01(0.56) degrees C per min per 100 g; P = 0.016). The perfusion flow rate required to reduce the temperature to less than 10 degrees C at a depth of 20 mm was lower in the UW group (P = 0.002). Kidneys flushed with HOC gained more weight than those flushed with UW (mean(s.d.) 50(8) versus 7(13) per cent; P = 0.002). Flushing with UW was associated with less histological injury but there were no significant differences in renal function parameters between the groups. CONCLUSION: UW cooled kidneys more slowly than HOC, but with no adverse effect on renal function. UW resulted in less oedema and histological injury than HOC.

Author information

Author/s: Kay, M D (MD); Hosgood, S A (SA); Bagul, A (A); Nicholson, M L (ML);

Affiliation: Transplant Group, Department of Infection, Immunity and Inflammation, University of Leicester, Leicester General Hospital, Leicester LE5 4PW, UK.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: The British journal of surgery (Br J Surg), published in England. (Language: eng)

Reference: 2009-Oct; vol 96 (issue 10) : pp 1215-21

Dates: Created 2009/10/05; Completed 2009/10/29;

PMID: 19787767, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenosine - pharmacology Allopurinol - pharmacology Animals Body Temperature Cold Temperature Glutathione - pharmacology Hypertonic Solutions - pharmacology Hypothermia, Induced - methods

Hypothermia, Induced - methods Insulin - pharmacology Kidney - physiology Kidney Transplantation Models, Biological Organ Preservation Solutions - pharmacology Raffinose - pharmacology Swine

Associated Chemicals: Hypertonic Solutions (0) ; Marshall's citrate solution (0) ; Organ Preservation Solutions (0) ; University of Wisconsin-lactobionate solution (0) ; Insulin (11061-68-0) ; Allopurinol (315-30-0) ; Raffinose (512-69-6) ; Adenosine (58-61-7) ; Glutathione (70-18-8)

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