Adenylyl cyclase type 5 contributes to corticostriatal plasticity and striatum-dependent learning.

Full Abstract

Dopamine (DA)-dependent corticostriatal plasticity is thought to underlie incremental procedural learning. A primary effector of striatal DA signaling is cAMP, yet its role in corticostriatal plasticity and striatum-dependent learning remains unclear. Here, we show that genetic deletion of a striatum-enriched isoform of adenylyl cyclase, AC5 knock-out (AC5KO), impairs two forms of striatum-dependent learning and corticostriatal synaptic plasticity. AC5KO mice were severely impaired in acquisition of a response strategy in the cross maze, a striatum-dependent task requiring a correct body turn to find a goal arm. In addition, AC5KO mice were impaired in acquisition of a motor skill, as assessed by the accelerated rotarod. Slice electrophysiology revealed a deficit in corticostriatal long-term depression (LTD) after high-frequency stimulation of tissue from AC5KO mice. LTD was rescued by activation of either presynaptic cannabinoid type 1 (CB(1)) receptors or postsynaptic metabotropic glutamate receptors (mGluRs), suggesting a postsynaptic role of AC5-cAMP, upstream of endocannabinoid release. In striatopallidal-projecting medium spiny neurons, DA D(2) receptors are negatively coupled to cAMP production, and activation of these receptors is required for endocannabinoid release and corticostriatal LTD. Recordings from striatopallidal neurons indicated that this is mediated by AC5, because coactivation of D(2) and mGluRs could induce LTD in wild-type but not in AC5KO neurons. To further examine the role of cAMP in corticostriatal plasticity, we elevated cAMP in striatal neurons of wild-type mice via the recording electrode. Under these conditions, corticostriatal LTD was eliminated. Together, these data suggest an AC5-cAMP-endocannabinoid-CB(1) signaling pathway in corticostriatal plasticity and striatum-dependent learning.

Author information

Author/s: Kheirbek, Mazen A (MA); Britt, Jon P (JP); Beeler, Jeff A (JA); Ishikawa, Yoshihiro (Y); McGehee, Daniel S (DS); Zhuang, Xiaoxi (X);

Affiliation: Department of Neurobiology and Anesthesia, The University of Chicago, Chicago, Illinois 60637, USA.

Grants: DA015918 (Agency:NIDA NIH HHS) ; DA022269 (Agency:NIDA NIH HHS) ; F31DA023340 (Agency:NIDA NIH HHS) ; F31MH076422 (Agency:NIMH NIH HHS) ; F32DA020427 (Agency:NIDA NIH HHS) ; GM067773 (Agency:NIGMS NIH HHS) ; HL059139 (Agency:NHLBI NIH HHS) ; MH66216 (Agency:NIMH NIH HHS) ; R01DA25875-2 (Agency:NIDA NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci), published in United States. (Language: eng)

Reference: 2009-Sep; vol 29 (issue 39) : pp 12115-24

Dates: Created 2009/10/01; Completed 2009/10/13;

PMID: 19793969, status: MEDLINE (last retrieval date: 10/13/2009, IMS Date: )

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