Research article summary (published 29 Sep 2009):

Increased expression of Mg(2+) transport proteins enhances the survival of Salmonella enterica at high temperature.

Full Abstract

Mg(2+) homeostasis is important for Salmonella pathogenesis. In Salmonella enterica, the transcription of the mgtA gene, which encodes a Mg(2+) transporter, is regulated by a Mg(2+)-sensing riboswitch [Cromie MJ, Shi Y, Latifi T, Groisman EA (2006) Cell 125:71-84]. In a genetic analysis of the determinants of thermotolerance in S. enterica serovar Typhimurium, we isolated the chr-1 mutation that increased the resistance of exponential phase cells to killing by high temperature. This mutation is a single base change in the mgtA riboswitch that causes high-level constitutive expression of mgtA. We showed that another mgtA riboswitch mutation, DeltaUTR(re-100), which had been constructed by Cromie et al., also confers similar increased thermotolerance. Surprisingly, the chr-1 mutation is located at a position that would not be predicted to be important for the regulatory function of the riboswitch. We obtained physiological evidence suggesting that the chr-1 mutation increases the cytosolic free Mg(2+) concentration. High-level expression of the heterologous MgtE Mg(2+) transport protein of Bacillus subtilis also enhanced the thermotolerance of S. enterica. We hypothesize that increased Mg(2+) accumulation might enhance thermotolerance by protecting the integrity of proteins or membranes, by mitigating oxidative damage or acting as an inducer of thermoprotective functions.

Author information

Author/s: O'Connor, Kathleen (K); Fletcher, Susanne A (SA); Csonka, Laszlo N (LN);

Affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A), published in United States. (Language: eng)

Reference: 2009-Oct; vol 106 (issue 41) : pp 17522-7

Dates: Created 2009/10/15; Completed 2009/11/03; Revised 2009/11/06;

PMID: 19805196, status: MEDLINE (last retrieval date: 11/9/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenosine Triphosphatases - genetics Bacterial Proteins - genetics Base Sequence Cation Transport Proteins - genetics Hot Temperature Magnesium - metabolism

Models, Molecular Molecular Sequence Data RNA, Bacterial - chemistry; genetics RNA, Ribosomal - chemistry; genetics Salmonella enterica - cytology; physiology Salmonella typhimurium - cytology; physiology

Associated Chemicals: Bacterial Proteins (0) ; Cation Transport Proteins (0) ; RNA, Bacterial (0) ; RNA, Ribosomal (0) ; Magnesium (7439-95-4) ; Adenosine Triphosphatases (EC 3.6.1.-) ; MgtB protein, Salmonella typhimurium (EC 3.6.1.-)

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