Research article summary (published 6 Oct 2009):

Gal4 turnover and transcription activation.

Full Abstract

Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function. Recently, Nalley et al. challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather 'locks in' to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al. used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes.

Author information

Author/s: Collins, Galen A (GA); Lipford, J Russell (JR); Deshaies, Raymond J (RJ); Tansey, William P (WP);

Affiliation: Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.

Journal and publication information

Publication Type: Comment; Journal Article

Journal: Nature (Nature), published in England. (Language: eng)

Reference: 2009-Oct; vol 461 (issue 7265) : pp E7; discussion E8

Dates: Created 2009/10/08; Completed 2009/10/20;

PMID: 19812621, status: MEDLINE (last retrieval date: 10/20/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

CommentOn: Nature. 2006 Aug 31;442(7106):1054-7. (PMID: 16929306)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Binding, Competitive - drug effects Chromatin Immunoprecipitation DNA-Binding Proteins - metabolism Estradiol - pharmacology Galactokinase - genetics Promoter Regions, Genetic - genetics Protein Binding - drug effects Receptors, Estrogen - agonists; chemistry; metabolism Reproducibility of Results

Reproducibility of Results Research Design Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics; metabolism Tamoxifen - analogs & derivatives; pharmacology Trans-Activators - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation

Associated Chemicals: DNA-Binding Proteins (0) ; GAL10 protein, S cerevisiae (0) ; GAL4 protein, S cerevisiae (0) ; Receptors, Estrogen (0) ; Saccharomyces cerevisiae Proteins (0) ; Trans-Activators (0) ; Transcription Factors (0) ; Tamoxifen (10540-29-1) ; Estradiol (50-28-2) ; 4-hydroxytamoxifen (68392-35-8) ; GAL1 protein, S cerevisiae (EC 2.7.1.6) ; Galactokinase (EC 2.7.1.6)

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