Research article summary (published 9 Oct 2009):

The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria.

Full Abstract

Cell-autonomous innate immune responses against bacteria attempting to colonize the cytosol of mammalian cells are incompletely understood. Polyubiquitylated proteins can accumulate on the surface of such bacteria, and bacterial growth is restricted by Tank-binding kinase (TBK1). Here we show that NDP52, not previously known to contribute to innate immunity, recognizes ubiquitin-coated Salmonella enterica in human cells and, by binding the adaptor proteins Nap1 and Sintbad, recruits TBK1. Knockdown of NDP52 and TBK1 facilitated bacterial proliferation and increased the number of cells containing ubiquitin-coated salmonella. NDP52 also recruited LC3, an autophagosomal marker, and knockdown of NDP52 impaired autophagy of salmonella. We conclude that human cells utilize the ubiquitin system and NDP52 to activate autophagy against bacteria attempting to colonize their cytosol.

Author information

Author/s: Thurston, Teresa L M (TL); Ryzhakov, Grigory (G); Bloor, Stuart (S); von Muhlinen, Natalia (N); Randow, Felix (F);

Affiliation: Medical Research Council Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Cambridge, UK.

Journal and publication information

Publication Type: Journal Article

Journal: Nature immunology (Nat Immunol), published in United States. (Language: eng)

Reference: 2009-Nov; vol 10 (issue 11) : pp 1215-21

Dates: Created 2009/10/20; Completed 2009/10/23;

PMID: 19820708, status: MEDLINE (last retrieval date: 10/23/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

Comments and Corrections

CommentIn: Nat Immunol. 2009 Nov;10(11):1137-9. (PMID: 19841643)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adaptor Proteins, Signal Transducing - immunology; metabolism Amino Acid Sequence Autophagy Hela Cells Humans Microtubule-Associated Proteins - immunology; metabolism Molecular Sequence Data Nuclear Proteins - immunology; metabolism

Protein Binding Protein-Serine-Threonine Kinases - immunology; metabolism Proteins - immunology; metabolism RNA Interference Salmonella Infections - immunology Salmonella enterica - immunology Ubiquitin - immunology; metabolism Ubiquitination

Associated Chemicals: Adaptor Proteins, Signal Transducing (0) ; C9orf156 protein, human (0) ; Microtubule-Associated Proteins (0) ; Nuclear Proteins (0) ; Proteins (0) ; SINTBAD protein, human (0) ; Ubiquitin (0) ; light chain 3, human (0) ; nuclear dot protein 52, human (0) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TBK1 protein, human (EC 2.7.11.1)

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