Osterix enhances BMSC-associated osseointegration of implants.

Full Abstract

Cellular and molecular events in osseointegration at the dental implant surface remain largely unknown. We hypothesized that bone marrow stromal cells (BMSCs) participate in this process, and that osterix (Osx) promotes implant osseointegration. To prove this hypothesis, we tracked double-labeled BMSCs in implantation sites created in nude mice transplanted with these cells. We also inserted implants into the femurs of our established transgenic mice after local administration of viruses encoding Osx, to determine the osteogenic effects of Osx. Immunohistochemical results demonstrated that BMSCs can recruit from peripheral circulation and participate in wound healing and osseointegration after implantation. Microcomputed tomography (microCT) analysis revealed an increased bone density at the bone-to-implant interface in the Osx group, and histomorphometric analysis indicated an elevated level of bone-to-implant contact in the Osx group. We concluded that exogenous BMSCs participate in the osseointegration after implantation, and that Osx overexpression accelerates osseointegration.

Author information

Author/s: Xu, B (B); Zhang, J (J); Brewer, E (E); Tu, Q (Q); Yu, L (L); Tang, J (J); Krebsbach, P (P); Wieland, M (M); Chen, J (J);

Affiliation: Division of Oral Biology, Tufts University School of Dental Medicine, One Kneeland Street, Boston, MA 02111, USA.

Grants: DE16710 (Agency:NIDCR NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Journal of dental research (J Dent Res), published in United States. (Language: eng)

Reference: 2009-Nov; vol 88 (issue 11) : pp 1003-7

Dates: Created 2009/10/15; Completed 2009/10/29;

PMID: 19828887, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Bone Density - drug effects; physiology
Bone Marrow Cells - drug effects; physiology
Dental Implants
Femur - drug effects; pathology; surgery
Male
Mice
Mice, Nude
Mice, Transgenic
Osseointegration - drug effects; physiology

Osseointegration - drug effects; physiology
Osteoblasts - drug effects; pathology
Osteocalcin - analysis
Osteogenesis - drug effects; physiology
Sialoglycoproteins - analysis
Stromal Cells - drug effects; physiology
Transcription Factors - analysis; pharmacology
Wound Healing - drug effects; physiology
X-Ray Microtomography
Zinc Fingers - physiology

Associated Chemicals: Dental Implants (0) ; Sialoglycoproteins (0) ; Transcription Factors (0) ; bone sialoprotein (0) ; osterix protein, mouse (0) ; Osteocalcin (104982-03-8)

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