Odontoblast TRP channels and thermo/mechanical transmission.

Full Abstract

Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

Author information

Author/s: Son, A R (AR); Yang, Y M (YM); Hong, J H (JH); Lee, S I (SI); Shibukawa, Y (Y); Shin, D M (DM);

Affiliation: Department of Oral Biology, Brain Korea 21 Project, Yonsei University College of Dentistry, 134 Sinchon-dong Seodaemon-gu, Seoul, 120-752, Korea.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of dental research (J Dent Res), published in United States. (Language: eng)

Reference: 2009-Nov; vol 88 (issue 11) : pp 1014-9

Dates: Created 2009/10/15; Completed 2009/10/29;

PMID: 19828889, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Mechanotransduction, Cellular - physiology
Mice
Mice, Inbred ICR
Odontoblasts - physiology
Osmosis
Signal Transduction - physiology
TRPM Cation Channels - analysis
TRPV Cation Channels - analysis
Temperature Sense - physiology
Transient Receptor Potential Channels - analysis; physiology

Associated Chemicals: ANKTM1 protein, mouse (0) ; Calcium Channels (0) ; TRPM Cation Channels (0) ; TRPM3 protein, mouse (0) ; TRPM8 protein, mouse (0) ; TRPV Cation Channels (0) ; TRPV1 protein, mouse (0) ; Transient Receptor Potential Channels (0) ; Trpv2 protein, mouse (0) ; Trpv3 protein, mouse (0) ; Trpv4 protein, mouse (0)

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