CCR5 down-regulates osteoclast function in orthodontic tooth movement.

Full Abstract

During orthodontic tooth movement, there is local production of chemokines and an influx of leukocytes into the periodontium. CCL5 plays an important role in osteoclast recruitment and activation. This study aimed to investigate whether the CCR5-receptor influences these events and, consequently, orthodontic tooth movement. An orthodontic appliance was placed in wild-type mice (WT) and CCR5-deficient mice (CCR5(-/-)). The expression of mediators involved in bone remodeling was evaluated in periodontal tissues by Real-time PCR. The number of TRAP-positive osteoclasts and the expression of cathepsin K, RANKL, and MMP13 were significantly higher in CCR5(-/-). Meanwhile, the expression of two osteoblastic differentiation markers, RUNX2 and osteocalcin, and that of bone resorption regulators, IL-10 and OPG, were lower in CCR5(-/-). Analysis of the data also showed that CCR5(-/-) exhibited a greater amount of tooth movement after 7 days of mechanical loading. The results suggested that CCR5 might be a down-regulator of alveolar bone resorption during orthodontic movement.

Author information

Author/s: Andrade, I (I); Taddei, S R A (SR); Garlet, G P (GP); Garlet, T P (TP); Teixeira, A L (AL); Silva, T A (TA); Teixeira, M M (MM);

Affiliation: Department of Orthodontics, Faculty of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar 500, CEP 31.270-901, Belo Horizonte, Minas Gerais, Brazil. ildeu_andrade(-atsign-)yahoo.com.br

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of dental research (J Dent Res), published in United States. (Language: eng)

Reference: 2009-Nov; vol 88 (issue 11) : pp 1037-41

Dates: Created 2009/10/15; Completed 2009/10/29;

PMID: 19828893, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acid Phosphatase - analysis
Animals
Biological Markers - analysis
Bone Remodeling - physiology
Bone Resorption - physiopathology
Cathepsins - analysis
Cell Movement - physiology
Chemokine CCL2 - analysis
Chemokine CCL3 - analysis
Chemokine CCL5 - analysis
Core Binding Factor Alpha 1 Subunit - analysis
Cysteine Endopeptidases - analysis
Cytokines - analysis
Down-Regulation - physiology

Associated Chemicals: Biological Markers (0) ; Ccl2 protein, mouse (0) ; Ccl3 protein, mouse (0) ; Ccl5 protein, mouse (0) ; Chemokine CCL2 (0) ; Chemokine CCL3 (0) ; Chemokine CCL5 (0) ; Core Binding Factor Alpha 1 Subunit (0) ; Cytokines (0) ; Isoenzymes (0) ; Osteoprotegerin (0) ; RANK Ligand (0) ; Receptors, CCR5 (0) ; Runx2 protein, mouse (0) ; Osteocalcin (104982-03-8) ; Interleukin-10 (130068-27-8) ; tartrate-resistant acid phosphatase (EC 3.1.3.-) ; Acid Phosphatase (EC 3.1.3.2) ; Cathepsins (EC 3.4.-) ; Cysteine Endopeptidases (EC 3.4.22.-) ; cathepsin K (EC 3.4.22.-) ; Matrix Metalloproteinase 13 (EC 3.4.24.-) ; Mmp13 protein, mouse (EC 3.4.24.-)

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